Vesicle size distributions measured by flow field-flow fractionation coupled with multiangle light scattering
Vesicle size distributions measured by flow field-flow fractionation coupled with multiangle light scattering
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DOI：
10.1016/s0006-3495(98)78033-6
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发表时间：
1998-06-01
影响因子：
3.4
通讯作者：
Monbouquette, HG
中科院分区：
文献类型：
--
作者：
Korgel, BA;van Zanten, JH;Monbouquette, HG
的分离方法，流场流分馏（流FFF），耦合在线与多角度激光光散射（MALLS）的同时测量的大小和浓度的连续洗脱的小泡从分馏器。这些大小和浓度的数据，收集作为洗脱时间的函数，可用于构建数量和质量加权囊泡大小分布。与大多数竞争，非侵入性的方法不同，这种流动FFF/MALLS技术能够测量囊泡大小分布，而无需单独的折射率检测器，使用粒度标准进行校准，或事先假设大小分布的形状。实验测得的大小分布的挤出和去污剂去除形成的囊泡是非高斯分布，并符合威布尔分布。流动FFF/MALLS表明，挤出和洗涤剂透析囊泡形成方法都可以产生接近大小的单分散群体，平均直径的标准偏差约为8%。相反，在分析双峰系统的动态光散射的分辨率相当低，流FFF/MALLS被证明是解决囊泡亚群的差异远小于两个因素的平均大小。
The separation method, flow field-flow fractionation (flow FFF), is coupled on-line with multiangle laser light scattering (MALLS) for simultaneous measurement of the size and concentration of vesicles eluting continuously from the fractionator. These size and concentration data, gathered as a function of elution time, may be used to construct both number-and mass-weighted vesicle size distributions. Unlike most competing, noninvasive methods, this flow FFF/MALLS technique enables measurement of vesicle size distributions without a separate refractive index detector, calibration using particle size standards, or prior assumptions about the shape of the size distribution. Experimentally measured size distributions of vesicles formed by extrusion and detergent removal are non-Gaussian and are fit well by the Weibull distribution. Flow FFF/MALLS reveals that both the extrusion and detergent dialysis vesicle formation methods can yield nearly size monodisperse populations with standard deviations of similar to 8% about the mean diameter. In contrast to the rather low resolution of dynamic light scattering in analyzing bimodal systems, flow FFF/MALLS is shown to resolve vesicle subpopulations that differ by much less than a factor of two in mean size.