Dietary omega-3 fatty acids normalize BDNF levels, reduce oxidative damage, and counteract learning disability after traumatic brain injury in rats
Dietary omega-3 fatty acids normalize BDNF levels, reduce oxidative damage, and counteract learning disability after traumatic brain injury in rats
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DOI：
10.1089/neu.2004.21.1457
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发表时间：
2004-10-01
影响因子：
4.2
通讯作者：
Gomez-Pinilla, F
中科院分区：
文献类型：
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作者：
Wu, A;Ying, Z;Gomez-Pinilla, F
Omega-3脂肪酸（即二十二碳六烯酸；DHA）调节信号转导和基因表达，保护神经元免于死亡。在这项研究中，我们检查了膳食补充omega - 3脂肪酸的能力，以帮助大脑应对创伤性损伤的影响。在进行轻度液体冲击损伤（FPI）之前，给大鼠喂食常规饮食或添加omega-3脂肪酸的实验饮食4周。FPI增加了Morris水迷宫大鼠的氧化应激和学习能力。这种类型的病变还降低了脑源性神经营养因子（BDNF）、突触素I和cAMP反应元件结合蛋白（CREB）的水平。已知BDNF通过调节突触素I和CREB促进突触传递和学习能力。在饮食中补充omega-3脂肪酸抵消了FPI所研究的所有影响，即BDNF和相关突触素I和CREB的正常化水平，减少了氧化损伤，并抵消了学习障碍。氧化应激的减少表明这种饮食对维持神经元功能和可塑性的机制有有益的影响。这些结果表明，富含omega-3的膳食补充剂可以防止创伤性脑损伤后可塑性降低和学习能力受损。
Omega-3 fatty acids (i.e., docosahexaenoic acid; DHA) regulate signal transduction and gene expression, and protect neurons from death. In this study we examined the capacity of dietary omega3 fatty acids supplementation to help the brain to cope with the effects of traumatic injury. Rats were fed a regular diet or an experimental diet supplemented with omega-3 fatty acids, for 4 weeks before a mild fluid percussion injury (FPI) was performed. FPI increased oxidative stress, and impaired learning ability in the Morris water maze. This type of lesion also reduced levels of brain-derived neurotrophic factor (BDNF), synapsin I, and cAMP responsive element-binding protein (CREB). It is known that BDNF facilitates synaptic transmission and learning ability by modulating synapsin I and CREB. Supplementation of omega-3 fatty acids in the diet counteracted all of the studied effects of FPI, that is, normalized levels of BDNF and associated synapsin I and CREB, reduced oxidative damage, and counteracted learning disability. The reduction of oxidative stress indicates a benevolent effect of this diet on mechanisms that maintain neuronal function and plasticity. These results imply that omega-3 enriched dietary supplements can provide protection against reduced plasticity and impaired learning ability after traumatic brain injury.