Chloride ion channels are associated with adherence of lymphatic endothelial cells
Chloride ion channels are associated with adherence of lymphatic endothelial cells
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DOI：
10.1006/mvre.1996.0058
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发表时间：
1996-11-01
影响因子：
3.1
通讯作者：
Campbell, TJ
中科院分区：
文献类型：
--
作者：
Martin, DK;Boneham, GC;Campbell, TJ
我们研究了氯离子通道作为淋巴管内皮细胞黏附和生长控制机制的一部分。氯通道抑制剂N-苯基邻氨基苯甲酸(0.1 mM)在接种于明胶包被的培养皿后的3小时内抑制了先前未贴壁细胞的初始黏附。钾通道阻滞剂4-氨基吡啶(1 MM)对细胞贴壁率无影响。当N-苯基邻氨基苯甲酸加入到生长3天的融合单层细胞中时，对细胞生长没有影响。这一点，以及N-苯基邻氨基苯甲酸不影响细胞超微结构的观察，表明质膜氯通道参与了淋巴管内皮细胞开始附着于底物时触发的初始信号级联反应。(C)1996年学术出版社。
We have examined the role of chloride ion channels as part of the control mechanism for adherence and growth of lymphatic endothelial cells. The chloride channel inhibitor N-phenylanthranilic acid (0.1 mM) inhibited the initial adherence of previously nonadherent cells in the period up to 3 hr following seeding onto gelatin-coated culture dishes. The potassium channel inhibitor 4-aminopyridine (1 mM) had no effect on the rate of cell adherence. N-Phenylanthranilic acid had no effect when added to confluent monolayers of cells that had been growing for 3 days. This, and the observation that N-phenylanthranilic acid did not affect the ultrastructure of the cells, suggested that plasma membrane chloride channels are involved in the initial signaling cascade triggered when lymphatic endothelial cells begin to adhere to a substrate. (C) 1996 Academic Press, Inc.