Co-occurrence of mcr-1, mcr-4 and mcr-5 genes in multidrug-resistant ST10 Enterotoxigenic and Shiga toxin-producing Escherichia coli in Spain (2006-2017)
Co-occurrence of mcr-1, mcr-4 and mcr-5 genes in multidrug-resistant ST10 Enterotoxigenic and Shiga toxin-producing Escherichia coli in Spain (2006-2017)
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DOI：
10.1016/j.ijantimicag.2018.03.022
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发表时间：
2018-07-01
影响因子：
10.8
通讯作者：
Blanco, Jorge
中科院分区：
文献类型：
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作者：
Garcia, Vanesa;Garcia-Menino, Isidro;Blanco, Jorge
粘菌素是一种抗菌多肽，通常用于控制和治疗由产肠毒素和产滋贺毒素大肠杆菌（ETEC和STEC）引起的新生儿和断奶后腹泻（PWD）疾病。质粒介导的粘菌素耐药基因mcr-1于2015年底首次被描述，此后，多项研究报告了其全球分布。此外，还发现了5种不同的mcr基因。本研究的目的是鉴定大肠杆菌对黏菌素的耐药性。在集约化养猪场中与PWD有关的大肠杆菌克隆群。在2006年至2017年在西班牙分离的186种ETEC和STEC中，76.9%对粘菌素具有耐药性。其中，102例为mcr-4携带者，37例为mcr-1携带者，5例为mcr-5携带者，5个分离株中同时出现mcr-1/mcr-4、mcr-1/mcr-5和mcr-4/mcr-5。检测到三种不同的mcr-4变体，包括本文描述的新mcr-4.4和mcr-4.5。有趣的是，克隆组ST 10-A（CH 11 -24）似乎是ma-4传播的主要原因。总之，我们的研究结果表明，养猪业是大肠杆菌耐多粘菌素的重要储存库。大肠杆菌、其他额外风险基因的携带者，如bla（ESBL）。这些食用动物可能正在传播多种耐药性的鸡尾酒，对人类健康构成令人担忧的威胁。(C)2018 Elsevier B. V.和国际化疗学会。All rights reserved.
Colistin is an antimicrobial polypeptide commonly employed for controlling and treating neonatal and post-weaning diarrhoea (PWD) diseases caused by Enterotoxigenic and Shiga toxin-producing Escherichia coli (ETEC and STEC). The plasmid-mediated colistin resistance gene, mcr-1 was first described in late 2015 and, since then, multiple studies have reported its global distribution. In addition, five different mcr genes have been identified. The aim of this study was to characterise the colistin-resistant E. coli clonal groups implicated in PWD in farms of intensive pig production. Of 186 ETEC and STEC isolated in Spain from 2006 to 2017, 76.9% showed resistance to colistin. Of those, 102 were mcr-4 carriers, 37 mcr-1 and 5 mcr-5, with co-occurrence of mcr-1/mcr-4, mcr-1/mcr-5 and mcr-4/mcr-5 in five isolates. Three different mcr-4 variants were detected, including the new mcr-4.4 and mcr-4.5 described here. Interestingly, the clonal group ST10-A (CH11-24) appears to be primarily responsible for the spread of ma-4. In summary, our results show that the pig industry is an important reservoir of colistin-resistant E. coli, carriers of other additional risk genes, such as bla(ESBL). These food-producing animals might be spreading a cocktail of multiple resistances, posing a worrisome threat to human health. (C) 2018 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.