Membrane water permeability related to antigen-presenting function of dendritic cells
Membrane water permeability related to antigen-presenting function of dendritic cells
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DOI：
10.1111/j.1365-2249.2008.03702.x
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发表时间：
2008-09-01
影响因子：
4.6
通讯作者：
Bai, C. X.
中科院分区：
文献类型：
--
作者：
Wang, G. F.;Dong, C. L.;Bai, C. X.
水通道蛋白5 （Aquaporin 5, AQP5）是一种参与多种生理过程的水通道蛋白，是膜渗透水通透性的主要决定因素。AQP5基因位于人类12q染色体，与哮喘主要易感位点位于同一区域。在本研究中，我们试图确定AQP5基因敲除是否对过敏原诱导的哮喘有一定影响。通过卵清蛋白（OVA）诱导的小鼠哮喘模型，我们发现与野生型（WT）小鼠相比，AQP5的缺失减少了哮喘的一些主要特征，如肺组织中炎症细胞浸润减少，细胞因子表达降低，支气管肺泡灌洗液中炎症细胞减少。由于发现小鼠气管内注射ova脉冲树突状细胞（dc）， AQP5基因敲除（AQP5(-/-)）的小鼠出现较少的炎症细胞。由于dc是主要的抗原提呈细胞，在抗原诱导哮喘中发挥重要作用，我们也探讨了基因敲除对dc的可能影响。令人惊讶的是，逆转录聚合酶链反应和荧光活化细胞分选分析显示，体内dc或体外骨髓单核细胞来源dc （mDC）表面存在高水平的AQP5。AQP5敲除小鼠的未成熟mDC （AQP5(-/-)）与WT相比，CD80和CD86的表达和内吞能力下降，但在脂多糖作用下成熟后，这种差异消失。aqp5介导的水跨膜可能在DCs的功能中起一定作用。然而，AQP5对dc功能影响的机制有待进一步研究。
Aquaporin 5 (AQP5) is one of the water channel proteins which participate in a wide array of physiological processes and are primary determinants of membrane osmotic water permeability. The AQP5 gene is located in human chromosome 12q, the same region as the location of the major asthma susceptibility loci. In this study we try to determine whether the AQP5 knock-out has some effect on allergen-induced asthma. With a mouse asthma model induced by ovalbumin (OVA), we found that deletion of AQP5 reduced some major characteristic features of asthma, such as less inflammation cell infiltration in lung tissues, lower cytokine expression and fewer inflammation cells in bronchoalveolar lavage fluids compared with those from wild-type (WT) mice. Because it was found that mice injected intratracheally with OVA-pulsed dendritic cells (DCs), the AQP5 gene knock-out (AQP5(-/-)) ones presented fewer inflammation cells. Because DCs are major antigen-presenting cells that play an important role in antigen-induced asthma, we also probed into the possible effect of gene knock-out on DCs. Surprisingly, reverse transcription-polymerase chain reaction and fluorescence activated cell sorter analysis showed high levels of AQP5 on the surface of DCs from in vivo or bone marrow monocyte-derived DCs (mDC) in vitro. Immature mDC from AQP5 knock-out mice (AQP5(-/-)) showed decreased expression of CD80 and CD86 and endocytosis ability compared with that from WT, but the difference disappeared after mDCs matured with lipopolysaccharide. AQP5-mediated water transmembrane may play some role in the function of DCs. However, the mechanism of the effect of AQP5 on the DCs' function needs to be investigated further.