Dynamic QTL and epistasis analysis on seedling root traits in upland cotton
Dynamic QTL and epistasis analysis on seedling root traits in upland cotton
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DOI：
10.1007/s12041-014-0341-8
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发表时间：
2014-04-01
影响因子：
1.5
通讯作者：
Hua, Jinping
中科院分区：
文献类型：
--
作者：
Liang, Qingzhi;Li, Pengbo;Hua, Jinping
根参与获取水分和养分，以及为植物提供结构支持。根系为发育分析提供了一个动态模型。利用高地棉F2群体和构建的遗传图谱，研究了苗期根系性状的数量性状位点（QTL）、动态条件QTL和上位性互作。共检测到37个影响根系性状的QTLs，35个基于根系性状（根尖、分叉、根长、表面积和体积）净增加量的动态条件QTLs，分别为移栽后10 d与移栽后5 d和移栽后15 d ~ 10 d。由于尚未同时检测到QTL和动态条件QTL，因此在根系的不同发育阶段存在明显的QTL和动态条件QTL的动态特征。进一步证实了QTL qRSA-chr 1 -1在5 ~ 10 DAT间隔时间内的加性和显性效应大量的根系性状双位点互作被无条件或动态检测到，少数上位性互作仅在5-10 DAT和10-15 DAT间隔时间内同时检测到，说明不同时期根系发育的遗传互作机制不同。动态条件QTL和上位性效应为理解高地棉根系动态提供了新的尝试，为根构型选择提供了线索。
Roots are involved in acquisition of water and nutrients, as well as in providing structural support to plant. The root system provides a dynamic model for developmental analysis. Here, we investigated quantitative trait loci (QTL), dynamic conditional QTL and epistatic interactions for seedling root traits using an upland cotton F-2 population and a constructed genetic map. Totally, 37 QTLs for root traits, 35 dynamic conditional QTLs based on the net increased amount of root traits (root tips, forks, length, surface area and volume) (i) after transplanting 10 days compared to 5 days, and (ii) after transplanting 15 days to 10 days were detected. Obvious dynamic characteristic of QTL and dynamic conditional QTL existed at different developmental stages of root because QTL and dynamic conditional QTL had not been detected simultaneously. We further confirmed that additive and dominance effects of QTL qRSA-chr1-1 in interval time 5 to 10 DAT (days after transplant) offset the effects in 10 to 15 DAT. Lots of two-locus interactions for root traits were identified unconditionally or dynamically, and a few epistatic interactions were only detected simultaneously in interval time of 5-10 DAT and 10-15 DAT, suggesting different interactive genetic mechanisms on root development at different stages. Dynamic conditional QTL and epistasis effects provide new attempts to understand the dynamics of roots and provide clues for root architecture selection in upland cotton.