Flight muscle-specific expression of act88F:: GFP in transgenic Culex quinquefasciatus Say (Diptera: Culicidae)
Flight muscle-specific expression of act88F:: GFP in transgenic Culex quinquefasciatus Say (Diptera: Culicidae)
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DOI：
10.1016/j.parint.2004.04.002
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发表时间：
2004-12-01
影响因子：
1.9
通讯作者：
Christensen, BM
中科院分区：
文献类型：
--
作者：
Allen, ML;Christensen, BM
本文描述了一种培养对丝虫传播具有抗性的库蚊的策略。该策略成功的一个必要条件是确定蚊子体内起作用的飞行肌肉特异性启动子。将黑腹果蝇act88F基因启动子区调控的GFP标记基因插入致倦库蚊基因组。孟德尔遗传学证实了这种转化。基因组Southern印迹与放射性标记探针杂交证实整个供体质粒整合到蚊子基因组中。转基因蚊子的GFP表达仅限于飞行肌。2004爱思唯尔爱尔兰有限公司版权所有。
A strategy to engineer a strain of Culex mosquitoes refractory to filarial transmission is described. A requirement for success of the strategy is identification of a flight muscle-specific promoter that functions in the mosquito. A GFP marker gene under the control of the promoter region of the Drosophila melanogaster act88F gene was inserted into the genome of Culex quinquefasciatus. Transformation was confirmed by Mendelian genetics. Hybridization of a genomic Southern blot to a radiolabeled probe verified that the entire donor plasmid integrated into the mosquito genome. GFP expression in the transgenic mosquitoes was restricted to the flight muscles. (C) 2004 Elsevier Ireland Ltd. All rights reserved.