A mitochondrial phosphatase PTPMT1 is essential for the early development of silkworm, Bombyx mori
A mitochondrial phosphatase PTPMT1 is essential for the early development of silkworm, Bombyx mori
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线粒体磷酸酶 PTPMT1 对于家蚕 Bombyx mori 的早期发育至关重要
DOI：
10.1016/j.bbrc.2020.07.124
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发表时间：
2020
影响因子：
3.1
通讯作者：
Togawa Toru
中科院分区：
文献类型：
--
作者：
Homma Yuri;Toga Kouhei;Daimon Takaaki;Shinoda Tetsuro;Togawa Toru
保幼激素(JH)在昆虫的发育、繁殖、多物性等生物学过程中起着重要的调控作用。在严格的时间调控下，JH主要由特定的JH生物合成酶在舌体(CA)中产生。在以前的研究中，我们从家蚕中发现了一个新的JH生物合成基因，蛋白酪氨酸磷酸酶线粒体1(PTPMT1)，它在CA中的表达几乎是唯一的，并且与JH在幼虫发育后期的合成活性有关。在本研究中，为了揭示PTPMT1在体内的功能，我们利用TALEN基因产生了PTPMT1基因敲除家蚕。在基因敲除突变体中，没有观察到JH活性缺陷的迹象。相反，PTPMT1基因敲除家蚕不仅在缺乏全酶活性的突变体中表现出胚胎致死、发育停滞和3龄致死，而且在缺乏线粒体易位信号的突变体中也表现出致死。此外，在PTPMT1基因敲除胚胎中，线粒体基因组编码的两个基因CyTB和ND3的表达减少，表明线粒体功能紊乱。这些结果表明，PTPMT1在脊椎动物的昆虫线粒体中发挥着保守的重要作用(S报道)。
Juvenile hormone (JH) plays important roles in the control of many biological processes in insects, such as development, reproduction, and polyphenism. JH is primarily produced in the corpora allata (CA) by specific JH biosynthetic enzymes under strict temporal regulation. In a previous study, we identified a novel putative JH biosynthetic gene,protein tyrosine phosphatase,mitochondrial 1(PTPMT1), from silkworm,Bombyx mori, whose expression is nearly exclusive in the CA and is correlated with JH synthetic activities during late larval development. In this study, to reveal the function of PTPMT1in vivo, we generatedPTPMT1knockout silkworms using TALEN. In the knockout mutants, no signs indicating defects in JH activity were observed. Instead,PTPMT1knockout silkworms showed embryonic lethality, developmental arrest, and 3rd-instar lethality not only in mutants lacking total enzymatic activity but also in mutants lacking mitochondrial translocation signals. Moreover, inPTPMT1knockout embryos, the expression of two genes encoded by the mitochondrial genome,CYTBandND3, was decreased, indicating a mitochondrial disorder. These results suggested that PTPMT1 plays conserved vital role(s) reported in vertebrates in insect mitochondria.