POSTTRANSFUSION NON-A, NON-B HEPATITIS IN CHIMPANZEES - PHYSICOCHEMICAL EVIDENCE THAT THE TUBULE-FORMING AGENT IS A SMALL, ENVELOPED VIRUS
POSTTRANSFUSION NON-A, NON-B HEPATITIS IN CHIMPANZEES - PHYSICOCHEMICAL EVIDENCE THAT THE TUBULE-FORMING AGENT IS A SMALL, ENVELOPED VIRUS
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DOI：
10.1016/0016-5085(85)90150-7
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发表时间：
1985-01-01
期刊：
影响因子：
29.4
通讯作者：
MAYNARD, JE
中科院分区：
文献类型：
--
作者：
BRADLEY, DW;MCCAUSTLAND, KA;MAYNARD, JE
通过静脉注射将与肝细胞胞质小管形成相关的输血后非甲型、非乙型肝炎实验性地传播给黑猩猩。接种经证实具有感染性的血浆，该血浆已沉淀并微滤或通过沉淀和速率区带结合进行纯化。因子 VIII 衍生的非 A、非 B 管形成剂显然会通过 80 nm 膜过滤器，并且可以通过使用纯化程序从受感染的血浆中回收它，假设非 A、非 B 管形成剂是小的有包膜病毒。结合已知的非甲非乙型小管形成剂对氯仿的敏感性及其与乙型肝炎病毒明显缺乏核酸同源性，显然人类输血后非甲型非乙型肝炎的至少一种病原体可能是一种小的有包膜RNA病毒。
Posttransfusion non-A, non-B hepatitis associated with the formation of hepatocyte cytoplasmic tubules was experimentally transmitted to chimpanzees by i.v. inoculation of a proven-infectious plasma that had been pelleted and microfiltrated or purified by a combination of pelleting and rate-zonal banding. A factor VIII-derived non-A, non-B tubule-forming agent evidently will pass through an 80-nm membrane filter and that it can be recovered from infected plasma by use of a purification procedure that assumes the non-A, non-B tubule-forming agent is a small, enveloped virus. In combination with the known sensitivity of the non-A, non-B tubule-forming agent to chloroform and its apparent lack of nucleic acid homology with hepatitis B virus, evidently at least 1 etiologic agent of human posttransfusion non-A, non-B hepatitis may be a small, enveloped RNA virus.