Expression of vascular endothelial growth factor, matrix metalloproteinase-9 and E-cadherin in the process of lymph node metastasis in oesophageal cancer.
Expression of vascular endothelial growth factor, matrix metalloproteinase-9 and E-cadherin in the process of lymph node metastasis in oesophageal cancer.
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DOI：
10.1038/sj.bjc.6690530
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发表时间：
1999-07
影响因子：
8.8
通讯作者：
Imamura M
中科院分区：
文献类型：
--
作者：
Sato F;Shimada Y;Watanabe G;Uchida S;Makino T;Imamura M
淋巴结转移是影响食管癌预后的独立因素。基质金属蛋白酶（MMPs）的表达和E-cadherin的减少与食管癌的淋巴结转移有关。我们以前报道过血管内皮生长因子（VEGF）的表达与淋巴结转移有关。本研究旨在探讨VEGF、MMP-9和E-cadherin在食管癌淋巴结转移过程中的表达是否稳定或发生变化。应用免疫组化方法检测食管鳞癌石蜡包埋标本中VEGF、MMP-9和E-cadherin的表达。我们使用两种不同的标准对134例原发性肿瘤和174例淋巴结转移进行了分类：淋巴结转移的存在或不存在[N（-）组]，以及转移的分期-早期（癌细胞<淋巴结的50%）或晚期（≥ 50%）-并比较了两组和两个分期之间的表达。N（-）组、N（+）组、早期组和晚期组的表达率分别为：VEGF（49%，74%，60%，33%）、MMP-9（76%，65%，95%，69%）和E-cadherin（49%，24%，55%，38%）。VEGF在晚期淋巴结转移中表达下调，而MMP-9在早期淋巴结转移中表达上调。E-cadherin表达在早期转移中有所恢复，但在晚期转移中再次受到抑制。提示VEGF、MMP-9和E-cadherin的表达在食管癌淋巴结转移过程中均有不同程度的变化，且变化方式不同。© 1999癌症研究运动
Lymph node metastasis is a strong independent prognostic factor for oesophageal cancer. The expression of matrix metalloproteinases (MMPs) and reduction of E-cadherin correlate with lymph node metastasis of oesophageal cancer. We previously reported that the expression of vascular endothelial growth factor (VEGF) is associated with lymph node metastasis. This study was designed to determine whether VEGF, MMP-9 and E-cadherin expression is stable or changes in the process of lymph node metastasis of oesophageal cancer. Using immunohistochemistry, we detected VEGF, MMP-9 and E-cadherin expression in paraffin-embedded specimens of oesophageal squamous cell carcinoma. We classified 134 primary tumours and 174 nodal metastases using two different criteria: the absence [Group N(–)] or presence [Group N(+)] of nodal metastasis, and the stage of metastasis – Early Stage (cancer cells < 50% of lymph node) or Late Stage (≥ 50%) – and compared the expression among two groups and among two stages. The expression rates of Group N(–), Group N(+), Early Stage and Late Stage are as follows: VEGF (49%, 74%, 60%, 33%), MMP-9 (76%, 65%, 95%, 69%) and E-cadherin (49%, 24%, 55%, 38%). VEGF expression was down-regulated in Late Stage lymph node metastasis, while MMP-9 expression was elevated in Early Stage metastasis. E-cadherin expression is restored somewhat in Early Stage metastasis, but suppressed again in Late Stage metastasis. These data suggest that the expression of VEGF, MMP-9 and E-cadherin each change in the process of lymph node metastasis in oesophageal cancer, and that the patterns of change are different. © 1999 Cancer Research Campaign