REPEATED ETHANOL ADMINISTRATION DECREASES PRODYNORPHIN BIOSYNTHESIS IN THE RAT HIPPOCAMPUS
REPEATED ETHANOL ADMINISTRATION DECREASES PRODYNORPHIN BIOSYNTHESIS IN THE RAT HIPPOCAMPUS
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DOI：
10.1016/0304-3940(92)90515-9
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发表时间：
1992-01-06
影响因子：
2.5
通讯作者：
PRZEWLOCKI, R
中科院分区：
文献类型：
--
作者：
PRZEWLOCKA, B;LASON, W;PRZEWLOCKI, R
采用原位杂交和放射免疫分析方法，分别研究了单次和重复（19天）灌胃乙醇对大鼠海马强啡肽原mRNA和α-新内啡肽水平的影响。 单次乙醇给药对这两个参数均无影响。 重复乙醇给药降低了免疫反应性（ir-）α-新内啡肽（约37%）和前吗啡肽mRNA（约37%）。57%）水平。 在最后一剂乙醇后两天，没有发现ir-α-新内啡肽组织含量的变化，而强啡肽原mRNA的水平仍然下降（约为2.5%）。44%）。 这些结果表明，反复乙醇引起海马强啡肽原的生物合成的长期持续下降，这种效果，可能发挥一定的作用，乙醇诱导的神经元功能的缺陷。
The effect of single and repeated (19 days) intragastric ethanol administration on prodynorphin mRNA and alpha-neoendorphin levels in the hippocampus of the rat was studied using the situ hybridization and RIA methods, respectively. Single ethanol administration had no effect on either of these two parameters. Repeated ethanol administration decreased both immunoreactive (ir-) alpha-neoendorphin (by ca, 37%) and prodymorphin mRNA (by ca. 57%) levels. Two days after the last dose of ethanol no changes in the ir-alpha-neoendorphin tissue content were found, whereas the level of prodynorphin mRNA remained decreased (by ca. 44%). These results suggest that repeated ethanol evokes a long-lasting decrease in the biosynthesis of hippocampal prodynorphin, this effect that may play some role in the ethanol-induced deficiency of neuronal functions.