Detection of NEO in muskmelon fruits inoculated with Fusarium sulphureum and its control by postharvest ozone treatment

Detection of NEO in muskmelon fruits inoculated with Fusarium sulphureum and its control by postharvest ozone treatment
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接种硫磺镰刀菌甜瓜果实中NEO的检测及采后臭氧处理的控制

DOI:
10.1016/j.foodchem.2018.01.149
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发表时间:
2018-07-15
期刊:
影响因子:
8.8
通讯作者:
Yong-Cai, Li
Yong-Cai, Li
中科院分区:
农林科学1区
文献类型:
--
作者:
Hua-Li, Xue;Yang, Bi;Yong-Cai, Li

文献摘要

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甜瓜的镰刀菌腐病是一种重要的采后腐病,不仅造成经济损失,而且还会造成霉菌污染。采用乙腈/水(84:16,v/v)萃取,PriboFast M270柱纯化,UPLC-MS/MS检测,建立了一种快速、灵敏的分析F. sulphum接种甜瓜中新茄醇(NEO)的方法。方法通过线性度(R = 0.9990)、回收率(88.1 ~ 136.9%)、精密度(RSD)进行验证
Fusarium rot of muskmelon, caused by Fusarium spp., is one of the most important postharvest decays, that not only causes economic losses but leads to trichothecenes contamination. A rapid and sensitive method was developed for neosolaniol (NEO) analysis in muskmelon inoculated with F. sulphureum, utilizing acetonitrile/water (84:16, v/v) extraction and PriboFast M270 columns purification and UPLC-MS/MS detection. Method validation was evaluated by linearity (R >= 0.9990), recovery (88.1-136.9%), precision (RSD