Genomic Characterization of a Nalidixic Acid-Resistant Salmonella Enteritidis Strain Causing Persistent Infections in Broiler Chickens.
Genomic Characterization of a Nalidixic Acid-Resistant Salmonella Enteritidis Strain Causing Persistent Infections in Broiler Chickens.
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DOI：
10.3389/fvets.2021.725737
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发表时间：
2021
影响因子：
3.2
通讯作者：
Borst LB
中科院分区：
文献类型：
--
作者：
Walker GK;Suyemoto MM;Hull DM;Gall S;Jimenez F;Chen LR;Thakur S;Crespo R;Borst LB
肠沙门氏菌亚种的强毒株。家禽携带的肠炎血清型（SE）可导致家禽患病，并可能导致人类食源性疾病。在同一场地内相隔一年饲养的两只肉鸡群在整个生长期均因 SE 引起的败血症而死亡。肉眼病变主要包括多发性浆膜炎，其次是卵黄球囊炎、关节炎、骨髓炎和脊柱炎。培养有病变的组织，产生 59 个 SE 分离株。通过 Rep-PCR 对这些菌株进行基因分型，然后对 15 个克隆分离株进行全基因组测序 (WGS)。该菌株 SE_TAU19 在肉鸡胚胎致死率测定中进一步表征了抗菌敏感性和毒力。 SE_TAU19 对萘啶酸和磺胺二甲氧嘧啶具有抗性，并且对胚胎具有毒性，所有受攻击的肉鸡胚胎在 3.5 天内死亡率为 100%。筛选 SE_TAU19 全基因组序列揭示了 7 个抗菌素耐药性 (AMR) 基因、120 个毒力基因和两个 IncF 质粒复制子，对应于单个血清型特异性 pSEV 毒力质粒。 pef、spv 和 rck 毒力基因定位于质粒序列组装。我们报告了来自持续感染肉鸡群的强毒 SE 菌株的表型和基因组特征，并提出了从分离株收集到基因组组装和序列分析的 SE 表征工作流程。有必要对肉鸡中的 SE 毒力进行进一步的 SE 监测和调查。
Virulent strains of Salmonella enterica subsp. enterica serovar Enteritidis (SE) harbored by poultry can cause disease in poultry flocks and potentially result in human foodborne illness. Two broiler flocks grown a year apart on the same premises experienced mortality throughout the growing period due to septicemic disease caused by SE. Gross lesions predominantly consisted of polyserositis followed by yolk sacculitis, arthritis, osteomyelitis, and spondylitis. Tissues with lesions were cultured yielding 59 SE isolates. These were genotyped by Rep-PCR followed by whole-genome sequencing (WGS) of 15 isolates which were clonal. The strain, SE_TAU19, was further characterized for antimicrobial susceptibility and virulence in a broiler embryo lethality assay. SE_TAU19 was resistant to nalidixic acid and sulfadimethoxine and was virulent to embryos with 100% mortality of all challenged broiler embryos within 3.5 days. Screening the SE_TAU19 whole-genome sequence revealed seven antimicrobial resistance (AMR) genes, 120 virulence genes, and two IncF plasmid replicons corresponding to a single, serovar-specific pSEV virulence plasmid. The pef, spv, and rck virulence genes localized to the plasmid sequence assembly. We report phenotypic and genomic features of a virulent SE strain from persistently infected broiler flocks and present a workflow for SE characterization from isolate collection to genome assembly and sequence analysis. Further SE surveillance and investigation of SE virulence in broiler chickens is warranted.
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影响因子：
14.9
作者：
NCBI Resource Coordinators
通讯作者：
NCBI Resource Coordinators
影响因子：
3.7
作者：
Cosentino S;Voldby Larsen M;Møller Aarestrup F;Lund O
通讯作者：
Lund O
影响因子：
4.4
作者：
Barroso Lima, Nicholas Costa;Tanmoy, Arif M.;Komurian-Pradel, Florence
通讯作者：
Komurian-Pradel, Florence
影响因子：
1.4
作者：
Borst, Luke B.;Suyemoto, M. Mitsu;Barnes, H. John
通讯作者：
Barnes, H. John
影响因子：
1.4
作者：
Fisher, ME;Trampel, DW;Griffith, RW
通讯作者：
Griffith, RW