PHYSICAL AND GENETIC ORGANIZATION OF PETITE AND GRANDE YEAST MITOCHONDRIAL-DNA .4. INVIVO TRANSCRIPTION PRODUCTS OF MITOCHONDRIAL-DNA AND LOCALIZATION OF 23 S RIBOSOMAL-RNA IN PETITE MUTANTS OF SACCHAROMYCES-CEREVISIAE
PHYSICAL AND GENETIC ORGANIZATION OF PETITE AND GRANDE YEAST MITOCHONDRIAL-DNA .4. INVIVO TRANSCRIPTION PRODUCTS OF MITOCHONDRIAL-DNA AND LOCALIZATION OF 23 S RIBOSOMAL-RNA IN PETITE MUTANTS OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0022-2836(74)90304-0
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发表时间：
1974-01-01
影响因子：
5.6
通讯作者：
FUKUHARA, H
中科院分区：
文献类型：
--
作者：
FAYE, G;KUJAWA, C;FUKUHARA, H
A series of isonuclear cytoplasmicpetite(ϱ−) mutants presenting various patterns of large deletions and retentions in theC321R—E514Rregion was used to study the functional organization of mitochondrial DNA.In vivotranscription products ofϱ−mitochondria were analysed by gel electrophoresis and, in an attempt to locate the 23 S ribosomal RNA gene in the mitochondrial genome, by RNA-DNA hybridization.The clones carrying theCRandEPgenetic markers contained the 23S mitochondrial rRNA gene and transcribed itin vivo. The clones keeping only theERmarker appeared to have at least a partial sequence of the 23 S rRNA gene; in one clone the whole 23 S rRNA sequence was still present and correctly transcribed. The clones keeping only theCRmarker region also had a part of the region sequence complementary to 23 S RNA.Noϱ−clone, in this series, possessed a DNA sequence corresponding to the 16 S rRNA.The possibility that the drug-resistance mutationE514Rmight be in the 23 S rRNA gene is discussed.
