A preliminary study of photoacoustic/ultrasound dual-mode imaging in melanoma using MAGE-targeted gold nanoparticles
A preliminary study of photoacoustic/ultrasound dual-mode imaging in melanoma using MAGE-targeted gold nanoparticles
复制标题
使用 MAGE 靶向金纳米粒子对黑色素瘤进行光声/超声双模式成像的初步研究
DOI：
10.1016/j.bbrc.2018.05.155
复制
发表时间：
2018-07-12
影响因子：
3.1
通讯作者：
Li, Pan
中科院分区：
文献类型：
--
作者：
Li, Xuelin;Wang, Dong;Li, Pan
纳米探针足够小，可以在脉管系统内循环，并且可以通过内皮间隙到达肿瘤组织，为精确的肿瘤监测和成像引导的分子水平抗肿瘤治疗提供了一种新的策略。光声和超声成像都是通过应用纳米探针进行癌症检测的非侵入性工具。本研究通过双乳法和碳二亚胺法成功制备了一种负载金纳米棒（Au-NRs）和液体全氟化碳（全氟己烷/PFH）的聚合物多功能纳米颗粒探针，并偶联针对黑色素瘤相关抗原（MAGE）的单克隆抗体（MAGE-1抗体），作为靶向光声/超声双模成像造影剂（MAGE- aupfh - nps）。体外细胞靶向实验显示，靶向组B16黑色素瘤细胞周围存在大量MAGE-Au-PFH-NPs。靶组的光声信号在体内明显增加，持续时间比非靶组长。在体外光声实验中，纳米探针的光声信号随Au-NR浓度的增加而增强。808 nm激光照射后超声观察增强信号。细胞毒性和生物相容性试验表明，MAGE-Au-PFH-NPs具有良好的生物安全性。MAGE-Au-PFH-NPs可以作为光声/超声双模造影剂，为无创靶向、监测和治疗肿瘤的新方法奠定基础。(C) 2018爱思唯尔公司版权所有。
Nanoprobes are small enough to circulate within the vasculature and can reach tumour tissues through the endothelial gap, providing a new strategy for accurate tumour monitoring and imaging-guided antitumour therapy at the molecular level. Both photoacoustic and ultrasonic imaging are noninvasive tools for cancer detection via the application of nanoprobes. In this study, a polymeric multifunctional nanoparticle probe loaded with gold nanorods (Au-NRs) and liquid perfluorocarbon (perfluorinated hexane/PFH) and conjugated to a monoclonal antibody (MAGE-1 antibody) to melanoma -associated antigens (MAGE) targeting melanoma was successfully prepared by the double emulsion and carbodiimide methods as a targeted photoacoustic/ultrasound dual-mode imaging contrast agent (MAGE-AuPFH-NPs). Cell-targeting experiments in vitro showed large amounts of MAGE-Au-PFH-NPs surrounding B16 melanoma cells in the targeted group. The photoacoustic signal in the targeted group was significantly increased, and the duration was longer than that in the untargeted group in vivo. The photo acoustic signal of the nanoprobes was enhanced with increased Au-NR concentration in the photoacoustic experiment in vitro. The enhanced signal was observed by ultrasound after 808-nm laser irradiation. A cytotoxicity and biocompatibility test showed that MAGE-Au-PFH-NPs exhibited good biological safety. The MAGE-Au-PFH-NPs can be used as a photoacoustic/ultrasound dual-mode contrast agent to lay the foundation for a promising new approach for the noninvasive targeting, monitoring and treatment of tumours. (C) 2018 Elsevier Inc. All rights reserved.