Intra-amygdala microinfusion of IL-6 impairs the auditory fear conditioning of rats via JAK/STAT activation
Intra-amygdala microinfusion of IL-6 impairs the auditory fear conditioning of rats via JAK/STAT activation
复制标题
杏仁核内微量输注 IL-6 通过 JAK/STAT 激活损害大鼠的听觉恐惧调节
DOI：
10.1016/j.bbr.2014.08.052
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发表时间：
2014-12-15
影响因子：
2.7
通讯作者：
Yang, Pingting
中科院分区：
文献类型：
--
作者：
Hao, Yongxin;Jing, He;Yang, Pingting
尽管越来越多的文献表明细胞因子参与精神障碍的病理生理学，但白介素 6 (IL-6) 在学习和记忆功能中的作用仍未得到解决。本研究旨在调查 IL-6 对杏仁核依赖性恐惧学习的影响。使用成年 Wistar 大鼠并进行听觉恐惧条件反射测试和药理学技术。数据显示，针对杏仁核的 IL-6 输注会剂量依赖性地损害条件性恐惧的获得和消除。此外，Western印迹分析的结果证实JAK/STAT通过IL-6处理被暂时激活磷酸化。此外，在IL-6治疗之前，用JSI-124（一种JAK/STAT3抑制剂）对大鼠进行治疗，结果显示IL-6诱导的恐惧调节损伤显着减少。综上所述，我们的结果表明，IL-6 可通过杏仁核调节大鼠在听觉恐惧条件反射中的学习行为。此外，杏仁核中的 JAK/STAT3 激活似乎在 IL-6 介导的大鼠听觉恐惧学习行为改变中发挥作用。 (C) 2014 Elsevier B.V. 保留所有权利。
Though accumulating literature implicates that cytokines are involved in the pathophysiology of mental disorders, the role of interleukin-6 (IL-6) in learning and memory functions remains unresolved. The present study was undertaken to investigate the effect of IL-6 on amygdala-dependent fear learning. Adult Wistar rats were used along with the auditory fear conditioning test and pharmacological techniques. The data showed that infusions of IL-6, aimed at the amygdala, dose-dependently impaired the acquisition and extinction of conditioned fear. In addition, the results in the Western blot analysis confirmed that JAK/STAT was temporally activated-phosphorylated by the IL-6 treatment. Moreover, the rats were treated with JSI-124, a JAK/STAT3 inhibitor, prior to the IL-6 treatment showed a significant decrease in the IL-6 induced impairments of fear conditioning. Taken together, our results demonstrate that the learning behavior of rats in the auditory fear conditioning could be modulated by IL-6 via the amygdala. Furthermore, the JAK/STAT3 activation in the amygdala seemed to play a role in the IL-6 mediated behavioral alterations of rats in auditory fear learning. (C) 2014 Elsevier B.V. All rights reserved.