FOCAL BRAIN ISCHEMIA IN THE RAT - METHODS FOR REPRODUCIBLE NEOCORTICAL INFARCTION USING TANDEM OCCLUSION OF THE DISTAL MIDDLE CEREBRAL AND IPSILATERAL COMMON CAROTID ARTERIES
FOCAL BRAIN ISCHEMIA IN THE RAT - METHODS FOR REPRODUCIBLE NEOCORTICAL INFARCTION USING TANDEM OCCLUSION OF THE DISTAL MIDDLE CEREBRAL AND IPSILATERAL COMMON CAROTID ARTERIES
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DOI：
10.1038/jcbfm.1988.88
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发表时间：
1988-08-01
影响因子：
6.3
通讯作者：
PULSINELLI, W
中科院分区：
文献类型：
--
作者：
BRINT, S;JACEWICZ, M;PULSINELLI, W
这篇文章描述了3年的经验局灶性新皮质缺血在三个大鼠品系。多组成年Wistar大鼠（n = 50）、Fischer 344大鼠（n = 31）和自发性高血压大鼠（n = 72）对大脑中远端（MCA）和同侧颈总动脉（CCA）进行永久性闭塞。24小时后处死小鼠，用苏木精和伊红染色冷冻脑切片，以区分梗死组织。每个切片的梗死体积用图像分析仪量化，总梗死体积用迭代程序计算所有间隔体积之和。自发性高血压大鼠（SHR）新皮质梗死体积最大，重现性最强。统计功率分析，以预测所需的动物数量，以检测梗死体积的25%或50%的变化。= 0.05和。beta。= 0.2表明只有SHR模型在所需动物数量上是可行的：即每组< 10只动物。在SHR应变中，远端MCA和同侧CCA的串联闭塞提供了一种简单的手术方法，可以引起具有可复制的地形和体积的大面积新皮质梗死。即使在SHR毒株中，动物间梗死体积的差异也表明，在每项研究中都需要随机对照，以确保对实验操作或药物治疗的准确评估。
This article describes a 3-year experience with focal neocortical ischemia in three rat strains. Multiple groups of adult Wistar (n = 50), Fischer 344 (n = 31), and spontaneously hypertensive (n = 72) rats were subjected to permanent occlusion of the distal middle cerebral (MCA) and ipsilateral common carotid arteries (CCA). Twenty-four hours later the animals were killed, and frozen brain sections were stained with hematoxylin and eosin to demarcate infarcted tissue. The infarct volume for each section was quantified with an image analyzer, and the total infarct volume was calculated with an iterative program that summed all interval volumes. Neocortical infarct volume was the largest and most reproducible in the spontaneously hypertensive rats (SHR). Statistical power analysis to project the numbers of animals necessary to detect a 25 or 50% change in infarct volume with .alpha. = 0.05 and .beta. = 0.2 revealed that only the SHR model was practical in terms of requisite animals: i.e., < 10 animals per group. Tandem occlusion of the distal MCA and ipsilateral CCA in the SHR strain provides a surgically simple method for causing large neocortical infarcts with reproducible topography and volume. The interanimal variability in infarct volume that occurs even in the SHR strain dictates that randomized, concomitant controls are necessary in each study to ensure the accurate assessment of experimental manipulations or pharmacologic therapies.