Globose basal cells are required for reconstitution of olfactory epithelium after methyl bromide lesion
Globose basal cells are required for reconstitution of olfactory epithelium after methyl bromide lesion
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DOI：
10.1002/cne.10642
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发表时间：
2003-05-19
影响因子：
2.5
通讯作者：
Schwob, JE
中科院分区：
文献类型：
--
作者：
Jang, WC;Youngentob, SL;Schwob, JE
尽管哺乳动物具有显著的再生能力，但在严重损伤的区域，哺乳动物嗅觉上皮的恢复可能会失败，随后重建为动脉瘤状呼吸道上皮(化生)。我们对比了甲基溴损伤后恢复为嗅觉的大鼠上皮区和呼吸上皮化生区域的细胞反应，以识别恢复受损上皮为嗅觉的干细胞。腹侧嗅觉上皮在损伤后尤其有可能发生化生，其斑块因暴露于甲基溴而脱细胞。相反，球状基底细胞(GBCs，以GBC-2染色标记)保留在周围腹区，均匀分布于背侧上皮，损伤后嗅觉持续完全恢复。在接下来的几天里，神经元会重新出现，但只出现在那些保存和增殖了GBCs的区域。相反，基底节细胞被破坏的部分上皮细胞部分由鲍曼腺细胞重新生长，这些细胞堆积在基底板上方。电子显微镜证实了腺细胞和球状基底细胞之间的相互作用。在损伤后14天，正在发生化生的区域被典型的呼吸道上皮细胞重新填充。由于水平的基底细胞从腹侧上皮的所有部分被消除，数据表明GBC-2(+)细胞是再生嗅神经上皮的最终责任。相反，GLA-13(+)细胞可产生呼吸道化生上皮，其中GBCs被清除。因此，我们支持这样的观点，即GBCs的一个亚群是嗅觉上皮的神经干细胞。(C)2003年Wiley-Liss，Inc.
Despite a remarkable regenerative capacity, recovery of the mammalian olfactory epithelium can fail in severely injured areas, which subsequently reconstitute as aneuronal respiratory epithelium (metaplasia). We contrasted the cellular response of areas of the rat epithelium that recover as olfactory after methyl bromide lesion with those undergoing respiratory metaplasia in order to identify stem cells that restore lesioned epithelium as olfactory. Ventral olfactory epithelium is at particular risk for metaplasia after lesion and patches of it are rendered acellular by methyl bromide exposure. In contrast, globose basal cells (GBCs, marked by staining with GBC-2) are preserved in surrounding ventral areas and uniformly throughout dorsal epithelium, which consistently and completely recovers as olfactory after lesion. Over the next few days, neurons reappear, but only in those areas in which GBCs are preserved and multiply. In contrast, parts of the epithelium in which GBCs are destroyed are repopulated in part by Bowman's gland cells, which pile up above the basal lamina. Electron microscopy confirms the reciprocity between gland cells and globose basal cells. By 14 days after lesion, the areas that are undergoing metaplasia are repopulated by typical respiratory epithelial cells. As horizontal basal cells are eliminated from all parts of the ventral epithelium, the data suggest that GBC-2(+) cells are ultimately responsible for regenerating olfactory neuroepithelium. In contrast, GLA-13(+) cells may give rise to respiratory metaplastic epithelium where GBCs are eliminated. Thus, we support the idea that a subpopulation of GBCs is the neural stem cell of the olfactory epithelium. (C) 2003 Wiley-Liss, Inc.