Development and cross-amplification of 16 polymorphic microsatellite markers in the Chestnut Bulbul Hemixos castanonotus
Development and cross-amplification of 16 polymorphic microsatellite markers in the Chestnut Bulbul Hemixos castanonotus
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DOI：
10.1007/s12686-010-9300-8
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发表时间：
2010
影响因子：
1.1
通讯作者：
Yuchun Wu;Fasheng Zou;Dongmei Yu;Zhiping Pan
中科院分区：
文献类型：
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作者：
Yuchun Wu;Fasheng Zou;Dongmei Yu;Zhiping Pan
本研究设计了一套能扩增板栗鳞茎动物Hemixos castanonotus的16个微卫星DNA位点的引物，并在一个近缘种灰鳞茎动物Hemixos flavala中进行了交叉扩增试验。共对30只栗头短脚鹎个体进行了基因分型。每个位点平均检测到6.5个等位基因（2-12个）。预期杂合度值范围为0.218至0.888。经Bonferroni校正后，有4个位点显著偏离Hardy-Weinberg平衡。未发现明显的连锁不平衡。结果表明，在灰椋鸟的16个基因座中，有15个基因座扩增成功，在15个个体中多态性丰富。这些标记将为栗短脚鹬的种群遗传学研究、保护和遗传改良提供有用的信息。
A set of primers to amplify 16 microsatellite DNA loci was developed for the Chestnut BulbulHemixos castanonotusand the cross-amplification of these loci was tested in a closely related species, the ashy bulbulHemixos flavala. A total of 30 Chestnut Bulbul individuals were genotyped. An average of 6.5 alleles per locus (2–12 alleles) was detected. The expected heterozygosity values ranged from 0.218 to 0.888. Four loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected. Fifteen of 16 loci were successfully amplified in the ashy bulbul, with rich polymorphism in 15 individuals tested. These markers will be useful in population genetics, conservation and phylogenies of the chestnut bulbul.