Damaging effects of water-borne cadmium chloride on DNA of lung cells of immature mice.
Damaging effects of water-borne cadmium chloride on DNA of lung cells of immature mice.
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DOI：
10.4238/2012.september.25.2
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发表时间：
2012-12
期刊：
影响因子：
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通讯作者：
X. Yang;Y. Ge;H. Zhang;H. Ning;J. Jiang;Y. Qi;Z. L. Wang
中科院分区：
文献类型：
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作者：
X. Yang;Y. Ge;H. Zhang;H. Ning;J. Jiang;Y. Qi;Z. L. Wang
我们研究了镉对未成年小鼠肺细胞DNA的影响。将小鼠随机分为4组：对照组、低剂量组（1/100 LD（50））、中剂量组（1/50 LD（50））和高剂量组（1/25 LD（50）），给予氯化镉或对照水40 d。从处死的小鼠收集的肺细胞用于通过彗星试验评估DNA损伤的程度。测量了拖尾细胞比率、DNA尾长、DNA彗星长、DNA尾矩、DNA橄榄尾矩和DNA在彗星尾中的百分比。染镉小鼠肺细胞拖尾率显著增加，低浓度组差异显著（P0.05），中、高剂量组差异均极显著（P < 0.01）。DNA损伤程度随镉浓度的增加而增加。我们的结论是，镉显着增加未成熟小鼠肺细胞DNA损伤的剂量依赖性的方式。
We investigated the effects of cadmium on lung cell DNA in immature mice. The mice were randomly divided into four groups: control group, low-dose group (1/100 LD(50)), middle-dose group (1/50 LD(50)), and high-dose group (1/25 LD(50)); they were supplied with cadmium chloride or control water for 40 days. Lung cells collected from sacrificed mice were used to evaluate the extent of DNA damage by comet assay. The ratio of tailing cells, DNA tail length, DNA comet length, DNA tail moment, DNA olive tail moment, and percentage of DNA in the comet tail were measured. The rate of tailing lung cells exposed to cadmium increased significantly; the low-concentration group had significantly (P 0.05), and the differences in middle- and high-dose groups were all highly significant (P < 0.01). The degree of DNA damage also increased with the increase of the cadmium concentrations. We conclude that cadmium significantly increases DNA damage in lung cells of immature mice in a dose-dependent manner.