Design, measurement and processing of region-specific DNA methylation assays: the mass spectrometry-based method EpiTYPER.
Design, measurement and processing of region-specific DNA methylation assays: the mass spectrometry-based method EpiTYPER.
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DOI：
10.3389/fgene.2015.00287
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发表时间：
2015
影响因子：
3.7
通讯作者：
Tobi EW
中科院分区：
文献类型：
--
作者：
Suchiman HE;Slieker RC;Kremer D;Slagboom PE;Heijmans BT;Tobi EW
EpiTYPER® 是一种基于质谱的亚硫酸氢盐测序方法，能够以定量和高通量的方式进行区域特异性 DNA 甲基化分析。该技术针对 100-600 个碱基对的基因组区域，主要以单核苷酸分辨率定量测量 DNA 甲基化水平。它特别适合大规模研究候选区域或验证全基因组 DNA 甲基化研究中的区域。在这里，我们详细描述了如何设计和执行 EpiTYPER 测量以及预处理数据，提供标准 EpiTYPER 协议中未提供的高质量测量的详细信息。
EpiTYPER® is a mass spectrometry-based bisulfite sequencing method that enables region-specific DNA methylation analysis in a quantitative and high-throughput fashion. The technology targets genomic regions of 100–600 base pairs and results in the quantitative measurement of DNA methylation levels largely at single-nucleotide resolution. It is particularly suitable for larger scale efforts to study candidate regions or to validate regions from genome-wide DNA methylation studies. Here, we describe in detail how to design and perform EpiTYPER measurements and preprocess the data, providing details for high quality measurements not provided in the standard EpiTYPER protocol.