Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy
Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy
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DOI：
10.1364/ol.29.002701
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发表时间：
2004-12-01
期刊：
影响因子：
3.6
通讯作者：
Cicerone, MT
中科院分区：
文献类型：
--
作者：
Kee, TW;Cicerone, MT
相干反斯托克斯拉曼散射（汽车）显微镜正在成为一种强大的成像材料和生物系统的方法，部分原因是它的非侵入性和选择性的化学敏感性。然而，其物种选择性成像的全部潜力受到有限光谱带宽的限制。最近增加的带宽是有希望的，但仍然是不够的水平，强大的组件歧视，将需要在一个化学复杂的环境中发现，例如，在细胞内和细胞外环境。我们展示了一个真正的宽带汽车成像仪器，我们用它来获取高光谱图像的振动光谱在带宽为2500厘米（-1），分辨率为13厘米（-1）。
Coherent anti-Stokes Raman scattering (CARS) microscopy is emerging as a powerful method for imaging materials and biological systems, partly because of its noninvasiveness and selective chemical sensitivity. However, its full potential for species-selective imaging is limited by a restricted spectral bandwidth. Recent increases in bandwidth are promising but still are not sufficient for the level of robust component discrimination that would be needed in a chemically complex milieu found, for example, in intracellular and extracellular environments. We demonstrate a truly broadband CARS imaging instrument that we use to acquire hyper-spectral images with vibrational spectra over a bandwidth of 2500 cm(-1) with a resolution of 13 cm(-1).