Impact of sewage sludges on Medicago truncatula symbiotic proteome

Impact of sewage sludges on Medicago truncatula symbiotic proteome
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DOI:
10.1016/j.phytochem.2004.04.037
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发表时间:
2004-06-01
期刊:
影响因子:
3.8
通讯作者:
Dumas-Gaudot, E
Dumas-Gaudot, E
中科院分区:
生物学2区
文献类型:
--
作者:
Bestel-Corre, G;Gianinazzi, S;Dumas-Gaudot, E

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研究了污水污泥对模式植物蒺藜苜蓿(Medicago truncatula)与丛枝菌根真菌Glomus mosseae和根瘤菌Sinorhizobium meliloti共生关系的影响。通过与对植物生长和根共生体显示出积极影响的对照污泥相比,富含多环芳烃或重金属的污泥是有害的。采用双向电泳和基质辅助激光解吸电离质谱技术检测和鉴定共生相关蛋白,并利用图像分析技术研究污水污泥对M.蒺藜共生蛋白质组(C)2004爱思唯尔有限公司保留所有权利。
The effects of sewage sludges were investigated on the symbiotic interactions between the model plant Medicago truncatula and the arbuscular mycorrhizal fungus Glomus mosseae or the rhizobial bacteria Sinorhizobium meliloti. By comparison to a control sludge showing positive effects on plant growth and root symbioses, sludges enriched with polycylic aromatic hydrocarbons or heavy metals were deleterious. Symbiosis-related proteins were detected and identified by two-dimensional electrophoresis and matrix-assisted laser desorption ionization mass spectrometry, and image analysis was used to study the effects of sewage sludges on M. truncatula symbiotic proteome. (C) 2004 Elsevier Ltd. All rights reserved.