EVIDENCE FOR A PROTECTIVE ROLE OF PULMONARY SURFACTANT PROTEIN-D (SP-D) AGAINST INFLUENZA-A VIRUSES
EVIDENCE FOR A PROTECTIVE ROLE OF PULMONARY SURFACTANT PROTEIN-D (SP-D) AGAINST INFLUENZA-A VIRUSES
复制标题
DOI：
10.1172/jci117323
复制
发表时间：
1994-07-01
影响因子：
15.9
通讯作者：
SASTRY, K
中科院分区：
文献类型：
--
作者：
HARTSHORN, KL;CROUCH, EC;SASTRY, K
我们测试了肺表面活性物质相关凝集素--表面活性蛋白A和D（SP-A和-D）--有助于对抗甲型流感病毒（IAV）的初始保护机制的假设。SP-D有效抑制几种UV菌株的血凝活性以及引起病毒聚集。SP-D增强IAV的中性粒细胞结合和中性粒细胞对病毒的呼吸爆发反应。当病毒与SP-D预孵育时，IAV暴露导致的神经功能障碍减少。这些作用中的每一个都是由SP-D的钙依赖性碳水化合物结合特性介导的。人和大鼠来源的天然SP-D制剂以及重组大鼠SP-D具有相似的活性。SP-A也能抑制IAV的血凝活性。我们以前曾报道过，相关的哺乳动物血清凝集素（甘露糖结合凝集素[MBL]和连接蛋白）具有类似的作用。SP-D在引起血凝抑制方面至少比SP-A或MBL强10倍。SP-D显示有助于人支气管肺泡灌洗液的有效抗IAV活性。这些结果表明，SP-D-单独，并与SP-A和吞噬细胞-构成了呼吸道内的IAV感染的自然免疫反应的重要组成部分。
We tested the hypothesis that pulmonary surfactant-associated lectins - surfactant proteins A and D (SP-A, and -D) - contribute to initial protective mechanisms against influenza A viruses (IAVs). SP-D potently inhibited hemagglutination activity of several strains of UV as well as causing viral aggregation. SP-D enhanced neutrophil binding of IAV and neutrophil respiratory burst responses to the virus. Neutrophil dysfunction resulting from IAV exposure was diminished when the virus was pre-incubated with SP-D. Each of these effects was mediated by the calcium-dependent carbohydrate-binding property of SP-D. Native SP-D preparations of both human and rat origin, as well as recombinant rat SP-D, had similar activity. SP-A also inhibited IAV hemagglutination activity. We have previously reported that related mammalian serum lectins (mannose-binding lectin [MBL] and conglutinin) have similar effects. SP-D was at least 10-fold more potent at causing hemagglutination inhibition than were SP-A or MBL. SP-D was shown to contribute to potent anti-IAV activity of human bronchoalveolar lavage fluid. These results suggest that SP-D-alone, and in conjunction with SP-A and phagocytic cells-constitutes an important component of the natural immune response to IAV infection within the respiratory tract.