MULTIPLICATION OF LOW AND HIGH CELL-CULTURE PASSAGED STRAINS OF TRANSMISSIBLE GASTROENTERITIS VIRUS IN ORGANS OF NEWBORN PIGLETS
MULTIPLICATION OF LOW AND HIGH CELL-CULTURE PASSAGED STRAINS OF TRANSMISSIBLE GASTROENTERITIS VIRUS IN ORGANS OF NEWBORN PIGLETS
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DOI：
10.1016/0378-1135(79)90033-6
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发表时间：
1979-01-01
影响因子：
3.3
通讯作者：
KUMAGAI, T
中科院分区：
文献类型：
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作者：
FURUUCHI, S;SHIMIZU, Y;KUMAGAI, T
比较了4株不同的传染性胃肠炎病毒(TGE)在仔猪体内的分布和持续时间。接种高传代TO-163菌株的仔猪在接种后的任何一天(DPI)均未表现出TGE的临床症状，而接种其他3个菌株之一的SH-14、SH-164或TO-16的仔猪则出现软便或腹泻。在后一例中，病毒主要从呼吸器官、淋巴结和消化道分离，但在接种TO-163毒株的仔猪消化道中确实检测到该病毒。在接种4个毒株中的1个毒株的仔猪中，从组织中恢复病毒的频率在4DPI之前最高，而在接种高传代毒株的仔猪中，从组织中恢复病毒的频率在此后显着降低。将TO-163株在新生仔猪体内连续传代7代。没有证据表明，随着传代的推进，病毒的致病性有所恢复。病毒的检测仅限于这些仔猪的淋巴和肺。在接种TO-163菌株的灵芝仔猪中，直到第4天，病毒恢复频繁，组织中的病毒滴度也很高。在部分仔猪的消化道中发现了高滴度的病毒，无一例出现TGE的临床症状。
Distribution and persistence of 4 different strains of transmissible gastroenteritis (TGE) virus in newborn piglets were compared. The piglets inoculated with high-passaged TO-163 strain did not show any clinical signs of TGE on any days postinoculation (DPI), but the piglets inoculated with 1 of the other 3 strains, SH-14, SH-164 or TO-16, had soft feces or diarrhea. In the latter cases, the virus was isolated mainly from respiratory organs, lymph nodes and digestive tract on any DIP, but was rearely detected in the digestive tract of piglets inoculated with the TO-163 strain. The frequency of virus-recovery from the tissues was the highest till 4 DPI in all of the piglets inoculated with 1 of the 4 virus strains and it was markedly reduced thereafter in the piglets inoculated with high-passaged strains. The TO-163 strain was subjected to serial passage in newborn piglets for 7 passages. There was no evidence of regained pathogenicity with advance in passage. Detection of virus was restricted to lymph nodes and lung of these piglets. In gnotobiotic piglets inoculated with the TO-163 strain, frequent virus recovery and high titers of virus from the tissues were obtained on up to the 4th DPI. The viruses in high titer were found in the digestive tract of some of the piglets; none showed any clinical signs of TGE.