C-Fos, Jun D and HSP72 immunoreactivity, and neuronal injury following lithium-pilocarpine induced status epilepticus in immature and adult rats
C-Fos, Jun D and HSP72 immunoreactivity, and neuronal injury following lithium-pilocarpine induced status epilepticus in immature and adult rats
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DOI：
10.1016/s0169-328x(98)00282-4
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发表时间：
1998-12-10
期刊：
影响因子：
--
通讯作者：
Nehlig, A
中科院分区：
文献类型：
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作者：
Dubé, C;André, V;Nehlig, A
为了跟踪Li-Pilo癫痫发作后10日龄（P10）、21日龄（P21）和成年大鼠神经元损伤、细胞活化和应激反应的成熟相关演变，我们分析了作为细胞活化标志物的c-Fos蛋白的表达，HSP 72免疫反应性作为应激反应，银染色用于评估20个选定脑区的神经元损伤。在癫痫发作后2小时测量的早期波c-Fos存在于大多数结构的动物在三个年龄段的研究，特别是在大脑皮层，海马和杏仁核。在癫痫发作后24 h测得的与神经元损伤相关的c-Fos晚波在P10大鼠脑中不存在，而主要存在于P21和成年大鼠的大脑皮层和海马。应激反应的表达，评估由HSP 72的免疫反应性在癫痫发作后24小时是不存在的P10大鼠脑和存在于内嗅皮层，杏仁核，海马和丘脑的P21和成年大鼠。Jun D在癫痫发作后24 h的表达是离散的，并存在于所有年龄的大多数脑区。癫痫发作后6 h通过银染色评估的神经元损伤在P10大鼠脑中非常离散，仅限于梨状皮质和内嗅皮质中的少数神经元。在老年动物中，大脑皮质、杏仁核、海马、外侧隔和丘脑中发生明显的神经元变性。因此，锂-匹罗卡品癫痫发作诱导的立即细胞活化在所有年龄段都存在，在P21和成年动物中仅转化为c-Fos的晚期波和HSP 72的表达，其中将存在广泛的细胞损伤。(C)1998 Elsevier Science B. V.保留所有权利。
In order to follow the maturation-related evolution of neuronal damage, cellular activation and stress response subsequent to Li-Pilo seizures in the 10- (P10), 21-day-old (P21) and adult rat, we analyzed the expression of the c-Fos protein as a marker of cellular activation, HSP72 immunoreactivity as the stress response and silver staining for the assessment of neuronal damage in 20 selected brain regions. The early wave of c-Fos measured at 2 h after the onset of seizures was present in most structures of the animals at the three ages studied and particularly strong in the cerebral cortex, hippocampus and amygdala. The late wave of c-Fos measured at 24 h after the onset of seizures and that was shown to correlate to neuronal damage was absent from the P10 rat brain, and present mainly in the cerebral cortex and hippocampus of P21 and adult rats. The expression of the stress response, assessed by the immunoreactivity of HSP72 at 24 h after the seizures was absent from the P10 rat brain and present in the entorhinal cortex, amygdala, hippocampus and thalamus of P21 and adult rats. The expression of Jun D at 24 h after the seizures was discrete and present in most brain regions at all ages. Neuronal injury assessed by silver staining at 6 h after the onset of seizures was very discrete in the brain of the P10 rat and limited to a few neurons in the piriform and entorhinal cortices. In older animals, marked neuronal degeneration occurred in the cerebral cortex, amygdala, hippocampus, lateral septum and thalamus. Thus the immediate cell activation induced by lithium-pilocarpine seizures which is present at all ages translates only into a late wave of c-Fos and the expression of HSP72 in P21 and adult animals in which there will be extensive cell damage. (C) 1998 Elsevier Science B.V. All rights reserved.