Immunological profile of resistance and susceptibility in naturally infected dogs by Leishmania infantum
Immunological profile of resistance and susceptibility in naturally infected dogs by Leishmania infantum
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DOI：
10.1016/j.vetpar.2014.08.022
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发表时间：
2014-10-15
影响因子：
2.6
通讯作者：
Reis, Alexandre Barbosa
中科院分区：
文献类型：
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作者：
de Almeida Leal, Gleisiane Gomes;Roatt, Bruno Mendes;Reis, Alexandre Barbosa
内脏利什曼病对公众健康影响很大，狗被认为是致病寄生虫婴儿利什曼原虫的主要家庭宿主。在这项研究中，通过使用流式细胞术分析细胞反应和血液学参数，对来自巴西流行区的 159 只自然感染婴儿乳杆菌的动物进行了评估。结果证实，疾病进展与贫血以及嗜酸性粒细胞、单核细胞和淋巴细胞减少有关。根据外周血免疫表型特征进行的免疫反应研究显示，无症状血清阳性（AD-II）和有症状血清阳性（SD）狗的T淋巴细胞CD5（+）及其亚群（CD4（+）和CD8（+））绝对数量下降，B淋巴细胞下降。当用婴儿乳杆菌可溶性抗原刺激时，AD-II 和 SD 组中的中性粒细胞显示出较高的干扰素 (IFN)-gamma(+) 合成，而根据聚合酶链反应测试（AD-I 组），婴儿乳杆菌感染呈阳性的无症状血清阴性狗中白细胞介素 (IL)-4(+) 的产生减少。在AD-II和SD组中，受刺激的淋巴细胞亚群（CD4（+）和CD8（+））在培养物中也表现出更多的IFN-γ（+）和IL-4（+）合成。这些结果表明，AD-II 和 SD 组的动物表现出混合的免疫反应（1 型和 2 型），而 AD-I 组的免疫特征与正常对照动物非常相似。 (C) 2014 Elsevier B.V. 保留所有权利。
Visceral leishmaniasis has a great impact on public health, and dogs are considered the main domestic reservoir of Leishmania infantum, the causal parasite. In this study, 159 animals naturally infected by L infantum from an endemic area of Brazil were evaluated through an analysis of cellular responses, using flow cytometry, and of the hematological parameters. The results confirmed that disease progression is associated with anemia and reductions in eosinophils, monocytes and lymphocytes. The investigation of the immune response, based on the immunophenotypic profile of peripheral blood, showed declines in the absolute numbers of T lymphocytes CD5(+) and their subsets (CD4(+) and CD8(+)) and a drop of B lymphocytes in asymptomatic seropositive (AD-II) and symptomatic seropositive (SD) dogs. Neutrophils, when stimulated with soluble antigen of L. infantum, showed higher synthesis of interferon (IFN)-gamma(+) in AD-II and SD groups, with decreased production of interleukin (IL)-4(+) in asymptomatic seronegative dogs positive for L. infantum infection based on polymerase chain reaction testing (AD-I group). In the AD-II and SD groups, subpopulations of stimulated lymphocytes (CD4(+) and CD8(+)) also exhibited greater synthesis of IFN-gamma(+) and IL-4(+) in culture. These results suggest that the animals of the AD-II and SD groups exhibited a mixed immune response (Type 1 and 2) and the AD-I group presenting an immune profile very similar to normal control animals. (C) 2014 Elsevier B.V. All rights reserved.