A survey of fermentation products and bacterial communities in corn silage produced in a bunker silo in China.
A survey of fermentation products and bacterial communities in corn silage produced in a bunker silo in China.
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DOI：
10.1111/asj.12076
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Chao Wang;Hongyan Han;Xu Gu;Zhu Yu;N. Nishino
中科院分区：
文献类型：
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作者：
Chao Wang;Hongyan Han;Xu Gu;Zhu Yu;N. Nishino
为评价我国玉米青贮饲料的管理现状，以北京市为研究对象，对北京市方圆500 km内14个玉米青贮场的青贮饲料发酵产物和细菌群落进行了分析。干物质（DM）含量的平均值低至250克/公斤，在玉米秸秆（ST）和全作物玉米（WC）青贮饲料，pH值平均分别为4.48和3.73。14种青贮饲料中只有3种表现出乳酸/乙酸比> 1.0，表明乙酸在发酵中占主导地位。虽然在大多数情况下1，2-丙二醇含量是边际的（25 g/kg DM.相比之下，3种St青贮饲料具有大量（> 10 g/kg DM）的丁酸，并且三种丁酸盐青贮饲料中的两种还具有高浓度的1-丙醇。变性梯度凝胶电泳分析表明，在14个青贮样品中，有10个样品的细菌群落相似。条带指示布氏乳杆菌L.在St和Wc青贮饲料中都发现了乙酸耐受菌和巴氏醋酸杆菌，这是青贮饲料样品中发现的高乙酸含量的原因。
To evaluate the current practice of corn silage management in China, samples of bunker-made silage were collected from 14 farms within a 500-km radius of Beijing for the analysis of fermentation products and bacterial communities. Mean values for dry matter (DM) content were as low as 250 g/kg in both corn stover (St) and whole crop corn (Wc) silages, and pH values averaged 4.48 and 3.73, respectively. Only three of the 14 silages exhibited a lactic-to-acetic acid ratio > 1.0, indicating that the presence of acetic acid was predominant in fermentation. Although 1,2-propanediol content was marginal in most cases ( 25 g/kg DM. In contrast, 3 St silages had large amounts (> 10 g/kg DM) of butyric acid, and two of the three butyrate silages also had high concentrations of 1-propanol. Denaturing gradient gel electrophoresis analysis demonstrated that the bacterial community appeared similar in 10 out of the 14 silage samples. Bands indicating Lactobacillus buchneri, L. acetotolerans and Acetobacter pasteurianus were found in both the St and Wc silages, accounting for the high acetic acid content found across silage samples.