A simple non-radioactive method for the simultaneous quantitative determination of stomach empyting and intestinal propulsion in the intact conscious rat.
A simple non-radioactive method for the simultaneous quantitative determination of stomach empyting and intestinal propulsion in the intact conscious rat.
复制标题
一种简单的非放射性方法，用于同时定量测定完整清醒大鼠的胃排空和肠道推进。
作者：
L. Borella;W. Lippmann
采用Amberlite颗粒，开发了一种简单的非放射性方法，用于同时评估完整禁食清醒大鼠的胃排空和肠推进。通过灌胃给予Amberlite颗粒，并在20或120 min后处死大鼠。测定胃和肠中颗粒的数量和百分比以及每个颗粒在小肠中的行进距离。采用小肠中领先颗粒行进的距离作为参数，以确定对肠推进的影响，而不依赖于胃排空活动。氯异松达明（s.c.），阿托品（s.c.），戊巴比妥（i.p.）和芝麻籽油（p.o.）以剂量相关方式抑制胃排空和肠推进。所有这些药物还引起小肠中颗粒的剂量相关位移，导致颗粒更倾向于头部分布。丙胺太林（s.c.）对胃排空有剂量相关的抑制作用，但对肠推进无抑制作用。卡巴胆碱（s.c.）增加胃排空率和小肠推进率。
A simple non-radioactive method for the simultaneous assessment of stomach emptying and intestinal propulsion in intact fasted conscious rats was developed employing Amberlite pellets. The Amberlite pellets were administered by gastric gavage and the rats were killed 20 or 120 min later. The number and percent of the pellets in the stomach and intestines and the distance travelled by each pellet in the small intestine were determined. The distance travelled by the leading pellet in the small intestine was employed as a parameter to determine effects on intestinal propulsion independent of the stomach emptying activity. Chlorisondamine (s.c.), atropine (s.c.), pentobarbital (i.p.) and sesame seed oil (p.o.) inhibited both stomach emptying and intestinal propulsion in a dose-related manner. All these agents also caused a dose-related displacement of the pellets in the small intestine which resulted in a more cephalad-oriented distribution of the pellets. Propantheline (s.c.) exerted a dose-related inhibition on the stomach emptying but not on intestinal propulsion. Carbachol (s.c.) increased both the rate of stomach emptying and that of propulsion in the small intestine.