Similar Responses of Circulating MicroRNAs to Acute High-Intensity Interval Exercise and Vigorous-Intensity Continuous Exercise.
Similar Responses of Circulating MicroRNAs to Acute High-Intensity Interval Exercise and Vigorous-Intensity Continuous Exercise.
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循环 MicroRNA 对急性高强度间歇运动和高强度连续运动的相似反应
DOI：
10.3389/fphys.2016.00102
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发表时间：
2016
影响因子：
4
通讯作者：
Ma JZ
中科院分区：
文献类型：
--
作者：
Cui SF;Wang C;Yin X;Tian D;Lu QJ;Zhang CY;Chen X;Ma JZ
据报道，高强度间歇运动(HIIE)比中、低强度运动更有利于身体适应。最近，越来越明显的是，循环中的miRNAs(c-miRNAs)可以区分由于运动持续时间、方式和类型的不同而施加的特定应激信号。本研究的目的是探讨HIIE是否优于大强度持续运动(VICE)，从而有助于开发有效的健康评估。26名年轻男性在运动前和HIIE或远距离配对卖淫后立即采集了血浆样本。用TaqMan低密度阵列(TLDA)初步测定了HIIE中的miRNA水平分布。用茎环定量逆转录聚合酶链式反应(RT-qPCR)验证差异表达的miRNAs水平。此外，对这些选择性c-miRNAs进行了检测。结果表明，HIIE后血浆中一些肌肉相关miRNAs水平，如miR-1、miR-133a、miR-133b和miR-206水平较安静时显著升高(P<；0.05)，HIIE时仅miR-1水平较安静时显著降低(P<；0.05)，而其他肌肉相关miRNAs水平在两种运动之间均无显著差异(P>；0.05)。此外，一些与组织相关或未知的原始miRNA水平，如miR-485-5p、miR-509-5p、miR-517a、miR-518f、miR-520f、miR-522、miR-553和miR-888，也显著高于安静时(P<；0.05)。然而，两种运动之间没有发现显著差异(P>；0.05)。总体而言，在这项研究中评估的耐力运动都能显著增加类似幅度的选择性c-miRNAs，这表明两种类型的耐力运动都有一般的应激过程。因此，对两种急性运动的相似反应可能表明这两种运动可以互换使用。还需要进一步的工作来揭示运动过程中c-miRNA周转变化背后的功能意义和信号机制。
High-intensity interval exercise (HIIE) has been reported to be more beneficial for physical adaptation than low-to-moderate exercise intensity. Recently, it is becoming increasingly evident that circulating miRNAs (c-miRNAs) may distinguish between specific stress signals imposed by variations in the duration, modality, and type of exercise. The aim of this study is to investigate whether or not HIIE is superior to vigorous-intensity continuous exercise (VICE), which is contributing to develop effective fitness assessment. Twenty-six young males were enrolled, and plasma samples were collected prior to exercise and immediately after HIIE or distance-matched VICE. The miRNA level profiles in HIIE were initially determined using TaqMan Low Density Array (TLDA). And the differentially miRNAs levels were validated by stem-loop quantitative reverse-transcription PCR (RT-qPCR). Furthermore, these selective c-miRNAs were measured for VICE. Our results showed that some muscle-related miRNAs levels in the plasma, such as miR-1, miR-133a, miR-133b, and miR-206 significantly increased following HIIE or VICE compared to those at rest (P < 0.05), and there was only a significant reduction in miR-1 level for HIIE compared to VICE (P < 0.05), while no significant differences were observed for other muscle-related miRNAs between both exercises (P > 0.05). In addition, some tissue-related or unknown original miRNA levels, such as miR-485-5p, miR-509-5p, miR-517a, miR-518f, miR-520f, miR-522, miR-553, and miR-888, also significantly increased (P < 0.05) in both exercises compared to rest. However, no significant differences were found between both exercises (P > 0.05). Overall, endurance exercise assessed in this study both led to significant increases in selective c-miRNAs of comparable magnitude, suggesting that both types of endurance exercise have general stress processes. Accordingly, the similar responses to both acute exercises likely indicate both exercises can be used interchangeably. Further work is needed to reveal the functional significance and signaling mechanisms behind changes in c-miRNA turnover during exercise.