The production of transforming growth factor-beta activity by rat granulosa cell cultures.
The production of transforming growth factor-beta activity by rat granulosa cell cultures.
复制标题
DOI：
10.1210/endo-124-3-1345
复制
发表时间：
1989-03
期刊：
影响因子：
4.8
通讯作者：
Inae Kim;D. W. Schomberg
中科院分区：
文献类型：
--
作者：
Inae Kim;D. W. Schomberg
我们检测了颗粒细胞（GC）是否分泌转化生长因子- β (TGF β)样活性，使用的细胞培养物是由diethylstilbestroll引发的雌性大鼠。我们的研究结果表明，在特定的GC培养基中发现了显著水平的活性和潜在的TGF β活性，通过以下评估：1)fsh诱导的大鼠GC分化增强，2)抗TGF β免疫球蛋白中和其活性，3)抑制水貂肺上皮细胞（CCl 64）的DNA合成，以及4)酸或热处理激活潜在的TGF β活性。当细胞接种在纤维连接蛋白包被板上时，TGF - β的产生更为明显。二乙基stilbestroll引发的未成熟大鼠、正常未成熟大鼠和成年大鼠的GC制剂对TGF - β的分泌水平没有差异。此外，与正常大鼠肾细胞（NRK 49-F）、人前列腺腺癌细胞（PC-3）或猪GC培养基相比，大鼠GC条件培养基含有更高的TGF - β活性。大鼠鞘/间质细胞培养基的活性与GC培养基相当。我们得出结论，大鼠GC制剂在体外分泌高水平的TGF β活性。结合以往的结果，这表明TGF β可能既是卵泡内的自分泌调节因子，也是旁分泌调节因子。此外，由于产生了高水平的TGF β活性，大鼠GC培养系统似乎是一个有用的实验模型，可以进一步探索TGF β产生与其作用之间的关系。
We have examined whether granulosa cells (GC) secrete transforming growth factor-beta (TGF beta)-like activity using cell cultures prepared from diethylstilbestrol-primed female rats. Our results indicate that a significant level of active as well as latent TGF beta activity is found in defined GC culture medium as assessed by 1) potentiation of FSH-induced differentiation of rat GC, 2) neutralization of its activity by anti-TGF beta immunoglobulin, 3) inhibition of DNA synthesis in mink lung epithelial cells (CCl 64), and 4) activation of latent TGF beta activity by either acid or heat treatment. TGF beta production was more pronounced when the cells were seeded on fibronectin-coated plates. There was no difference in the level of TGF beta secretion by GC preparations derived from either diethylstilbestrol-primed immature or normal immature rats or adult rats. Furthermore, rat GC-conditioned medium contained much more TGF beta activity than medium from normal rat kidney cells (NRK 49-F), human prostatic adenocarcinoma cells (PC-3), or porcine GC. Rat thecal/interstitial cell culture medium contained activity comparable to that of GC medium. We conclude that rat GC preparations secrete a high level of TGF beta activity in vitro. Taken together with previous results, this indicates the possibility that TGF beta may be an autocrine regulator as well as a paracrine one within the ovarian follicle. Moreover, because of the high level of TGF beta activity produced, the rat GC culture system appears to be a useful experimental model for further exploring relationships between TGF beta production and its action.