Cloning and developmental regulation of a novel member of the insulin-like gene family in Caenorhabditis elegans.
Cloning and developmental regulation of a novel member of the insulin-like gene family in Caenorhabditis elegans.
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秀丽隐杆线虫胰岛素样基因家族新成员的克隆和发育调控。
DOI：
10.1006/bbrc.1998.9164
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发表时间：
1998
影响因子：
3.1
通讯作者：
Warden,CH
中科院分区：
文献类型：
--
作者：
Gregoire,FM;Chomiki,N;Kachinskas,D;Warden,CH
秀丽隐杆线虫 (C. elegans) 的衰老、代谢和脂肪积累受到 DAF-2（一种假定的胰岛素样受体）突变的影响。最近从 C 中鉴定出了 10 个假定的胰岛素样基因。线虫基因组数据库。然而，尚不清楚这些基因是否是人胰岛素的直系同源物，因为它们缺乏 C 肽二元氨基酸蛋白水解位点。我们已经鉴定并测量了 C 的两个新成员发育过程中的 mRNA 表达。 elegansinsulin 样基因家族。我们还报告了其中一种胰岛素样蛋白 1 (ILP1) 的序列特征和基因结构。我们重点关注 ILP1 的表征，因为它具有与其作为 DAF-2 胰岛素样受体的候选胰岛素配体一致的结构特征。例如，ILP1 具有推定的 C 肽，两侧为二元氨基酸，具有保守的半胱氨酸残基，可以在 A 链和 B 链之间提供二硫键，并且具有两个内含子。 Northern印迹分析显示ILP1mRNA在胚胎中表达水平非常高，并且在出生后发育过程中很早就被下调，这表明它可能影响胚胎发育，但不影响Dauer形成。我们还发现了一种新型胰岛素样生长因子 1 样蛋白 (T28B8/IGF-I)，其表现出与 ILP1 非常不同的发育表达谱。我们的结果与异常大且复杂的 C 成员的假设是一致的。线虫胰岛素样蛋白家族表现出复杂且可能多余的作用。
Aging, metabolism and fat accumulation inCaenorhabditis elegans(C. elegans) are influenced by mutations in DAF-2, a putative insulin-like receptor. Ten putative insulin-like genes have been recently identified from theC. elegansgenome database. However, it is unclear if these genes are orthologues of human insulin since they lack the C-peptide dibasic amino acid proteolysis sites. We have identified and measured mRNA expression during development of two novel members of theC. elegansinsulin-like gene family. We also report the sequence characterization and gene structure for one of these, the insulin-like protein-1 (ILP1). We focused onILP1characterization because it has structural features consistent with its being a candidate insulin ligand for the DAF-2 insulin-like receptor. For example, ILP1 has a putative C-peptide flanked by dibasic amino acids, exhibits conserved cysteine residues that could provide disulfide bonds between the A and B chains, and has two introns. Northern blot analysis revealed thatILP1mRNA is expressed at very high levels in embryos and is downregulated very early during postnatal development, suggesting that it may influence embryonic development, but not Dauer formation. We also identified a novel insulin-like growth factor-1-like protein (T28B8/IGF-I) that exhibits a very different developmental expression profile than ILP1. Our results are consistent with the hypothesis that members of the unusually large and complexC. elegansinsulin-like protein family exhibit complex and perhaps redundant roles.
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DOI：
--
发表时间：
1976
期刊：
Journal of Biochemistry (Tokyo)
影响因子：
--
作者：
T. Morita;S. Iwanaga;T. Suzuki
通讯作者：
T. Suzuki
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DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Higgins,DL;Mann,KG
通讯作者：
Mann,KG
尿激酶对精氨酸氯甲基酮肽亲和标记的敏感性。
DOI：
10.1016/0005-2744(79)90078-0
发表时间：
1979
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Charles Kettner;Elliott Shaw
通讯作者：
Elliott Shaw
影响因子：
6.7
作者：
P. Holvoet;Hugo Cleemput;D. Collen
通讯作者：
P. Holvoet;Hugo Cleemput;D. Collen
影响因子：
--
作者：
M. K. Pangburn
通讯作者：
M. K. Pangburn