African swine fever:: Expression of interleukin-1 alpha and tumour necrosis factor-alpha by pulmonary intravascular macrophages
African swine fever:: Expression of interleukin-1 alpha and tumour necrosis factor-alpha by pulmonary intravascular macrophages
复制标题
DOI：
10.1053/jcpa.2001.0543
复制
发表时间：
2002-02-01
影响因子：
0.8
通讯作者：
Sierra, MA
中科院分区：
文献类型：
--
作者：
Carrasco, L;Núñez, A;Sierra, MA
来确定。在急性非洲猪瘟（ASF）中，肺水肿的出现与肺血管内巨噬细胞（pim）的病毒复制和细胞因子表达的关系，用ASF病毒（西班牙70株）肌肉注射14头猪，并在接种后1-7天两两杀死。对肺标本进行免疫组织化学和超微结构检查。采用白细胞介素-1 α、肿瘤坏死因子- α、ASF病毒抗原（Vp73）和骨髓标志物（SWC3）抗体进行免疫组化研究。病毒复制主要在pim中观察到，同时表现出强烈的激活。伴有il -1 α和tnf - α的表达。间质性水肿、中性粒细胞滞留和间隔毛细血管纤维蛋白微血栓的发生与感染的pim细胞因子的高表达一致。肺泡巨噬细胞在ASF感染后未表现出细胞因子表达的显著变化，并且仅在这些细胞中检测到病毒复制的比例很低。(C) 2002 Elsevier Science Ltd.
To determine. in the acute form of African swine fever (ASF), the relationship between the appearance of pulmonary oedema and viral replication and expression of cytokines by pulmonary intravascular macrophages (PIMs), 14 pigs were inoculated intramuscularly with ASF virus (strain Espana' 70) and killed in pairs on days 1-7 post-inoculation. Samples of lung were examined immunohistochemically and ultrastructurally. The immunohistochemical study was carried out with antibodies against interleukin-1 alpha IL-1alpha, tumour necrosis factor-alpha TNF-alpha), viral antigen of ASF (Vp73) and a myeloid marker (SWC3). Viral replication was observed mainly in PIMs, which at the same time showed intense activation. accompanied by the expression of IL-1alpha and TNF-alpha. The occurrence of interstitial oedema, neutrophil sequestration and fibrin microthrombi in septal capillaries coincided with high degrees of cytokine expression by infected PIMs. Alveolar macrophages did not show a significant change in cytokine expression as a result of ASF infection, and viral replication was detected in only a low percentage of these cells. (C) 2002 Elsevier Science Ltd.