Repeated assessment of larynx compound muscle action potentials using a self-sizing cuff electrode around the vagus nerve in experimental rats
Repeated assessment of larynx compound muscle action potentials using a self-sizing cuff electrode around the vagus nerve in experimental rats
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DOI：
10.1016/j.jneumeth.2011.04.007
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发表时间：
2011-06-15
影响因子：
3
通讯作者：
Boon, Paul
中科院分区：
文献类型：
--
作者：
El Tahry, Riem;Mollet, Lies;Boon, Paul
理由：迷走神经刺激（VNS）是难治性癫痫患者的辅助治疗方法。在超过30%的患者中，VNS没有治疗效果。本研究的目的是寻找一个客观参数，可以作为有效刺激迷走神经的指标。方法：记录迷走神经、喉返神经和喉肌对VNS的电生理反应。利用神经病变和肌肉松弛剂寻找电生理反应的来源。植入一个用于慢性刺激和记录的袖带电极，用于慢性记录植入后vns诱导的电生理反应。在2个月的随访期间，每天测定剂量-反应曲线。结果：VNS在刺激开始后约3ms诱导电生理反应。这种反应被确定为喉复合动作电位（LCMAP）， 11/21大鼠术后可立即记录LCMAP，而其他10/21大鼠术后平均需要25天的恢复期。一旦记录到LCAMP， LCMAP的潜伏期和剂量反应曲线的总体特征在整个随访期间保持稳定。结论：本研究为迷走神经的激活提供了客观的电生理参数。LCAMP可能表明植入后迷走神经的恢复，这可能有助于确定何时可以开始VNS治疗。LCAMP在癫痫动物模型的VNS物化实验中具有一定的应用价值。(C) 2011 Elsevier B.V.版权所有
Rationale: Vagus nerve stimulation (VNS) is an adjunctive treatment for patients with refractory epilepsy. In more than 30% of the patients VNS has no therapeutic effect. The goal of this study was to find an objective parameter that can be used as an indicator of effective stimulation of the vagus nerve.Methods: The electrophysiological response to VNS was recorded from the vagus nerve, recurrent laryngeal nerve and larynx muscles. Nerve lesions and muscle relaxing agent were used to find the source of the electrophysiological response. A cuff-electrode for chronic stimulation and recording was implanted for chronic recording of the VNS-induced electrophysiological response after implantation. Dose-response curves were determined daily during a follow-up period of 2 months.Results: VNS induced an electrophysiological response around 3 ms after start of the stimulation. This response was identified as a larynx compound action potential (LCMAP) LCMAP could be recorded immediately after surgery in 11/21 rats, while in the other 10/21 rats, a recovery period with an average of 25 days was required. Once the LCAMP could be recorded, the latency and overall characteristics of the doses response curves of the LCMAP remained stable during the entire follow-up period.Conclusions: In this study, we provide an objective electrophysiological parameter for vagus nerve activation. LCAMP may indicate recovery of the vagus nerve after implantation, which may help to determine when uptitration of VNS therapy can be initiated. LCAMP could be of value in future experiments for objectification of VNS in animal models for epilepsy. (C) 2011 Elsevier B.V. All rights reserved.