ENVIRONMENTAL EFFECTS ON LIPIDS OF MYCOBACTERIUM-PHLEI ATCC354
ENVIRONMENTAL EFFECTS ON LIPIDS OF MYCOBACTERIUM-PHLEI ATCC354
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DOI：
10.1139/m77-002
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发表时间：
1977-01-01
影响因子：
2.8
通讯作者：
VENKITASUBRAMANIAN, TA
中科院分区：
文献类型：
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作者：
DHARIWAL, KR;CHANDER, A;VENKITASUBRAMANIAN, TA
M.的磷脂Phlei ATCC 354中的磷脂酰肌醇是心磷脂、磷脂酰乙醇胺、三酰化和四酰化二甘露磷酸肌醇和四酰化五甘露磷酸肌醇。与以前的报告相反，monomannophosphoinositide并不明显。随着培养基中N含量的增加，总脂质呈线性下降。观察到的负相关关系与C供应中的媒体。在氮含量有限的培养基上生长的细胞含有更多的饱和脂肪酸，而在碳含量有限的培养基上生长的细胞含有更多的不饱和脂肪酸。磷脂酰乙醇胺没有显着变化，导致从改变培养基中的C和N供应。磷脂酰肌醇甘露糖苷是甘油生长的细胞中的主要磷脂，但在葡萄糖生长的细胞中的磷脂酰肌醇甘露糖苷和心磷脂的浓度是相同的。细胞甘油含量随C供应量的增加而增加，随N供应量的增加而减少。在37 ℃下，磷脂酰肌醇甘露糖苷是主要的磷脂成分。而心磷脂是在27 ℃生长的细胞中的主要磷脂。C.在脂肪酸中，随着生长温度从37 ℃降低到27 ℃，观察到棕榈酸显著降低，棕榈油酸和油酸伴随增加。C.在各种培养条件下，某些脂质成分有优先产生。
Phospholipids of M. phlei ATCC 354 are cardiolipin, phosphatidyl ethanolamine, tri- and tetra-acylated dimannophosphoinositide and tetraacylated pentamannophosphoinositide. Contrary to a previous report, monomannophosphoinositide was not evident. A linear decrease in total lipid was observed with an increase in the N content of the medium. An inverse relationship was observed with the C supply in the medium. Cells grown on a medium with limited N contained more saturated fatty acids, whereas cells grown on a medium with limited C contained more unsaturated fatty acids. No significant change in phosphatidyl ethanolamine resulted from changing the C and N supply in the medium. Phosphatidylinositol mannosides were the major phospholipids in glycerol-grown cells, but in glucose-grown cells concentrations of phosphatidylinositol mannosides and cardiolipin were the same. Glyceride content of cells increased with an increase in C supply and decreased with an increase in N supply in the medium. Phosphatidylinositol mannosides were the major phospholipid component at 37.degree. C, whereas cardiolipin was the major phospholipid in cells grown at 27.degree. C. Among fatty acids, a considerable decrease in palmitic acid with a concomitant increase in palmitoleic and oleic acid was observed with a decrease in growth temperature from 37 to 27.degree. C. There is a preferential production of certain lipid constituents under various cultural conditions.