A Subpopulation of Serotonergic Neurons That Do Not Express the 5-HT1A Autoreceptor
A Subpopulation of Serotonergic Neurons That Do Not Express the 5-HT1A Autoreceptor
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DOI：
10.1021/cn300157s
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发表时间：
2013-01-01
影响因子：
5
通讯作者：
Fernandez, Sebastian P.
中科院分区：
文献类型：
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作者：
Kiyasova, Vera;Bonnavion, Patricia;Fernandez, Sebastian P.
5-HT 神经元在后脑中按拓扑结构组织，与抑郁症和焦虑症等精神疾病的病因和治疗有关。早期研究表明，中缝 5-HT 神经元是一个同质群体，表现出相似的电特性，并且由 5-HT1A 自身受体介导反馈抑制。我们利用 ePet1-eGFP 和 5-HT1A-iCre/R26R 小鼠的组织化学技术表明 5-HT 神经元亚群不表达体树突 5-HT1A 自受体 mRNA。 In addition, we performed patch-clamp recordings followed by single-cell PCR in ePet1-eGFP mice.从位于中缝背侧、外侧和中缝的 134 个记录的 5-HT 神经元中，我们发现 22 个细胞缺乏 5-HT1A mRNA 表达，均匀分布在中缝亚区。我们比较了这些神经元类型的细胞特征，发现被动膜特性和一般兴奋性没有差异。然而，当注射大去极化电流时，5-HT1A 阴性神经元会激发更多的动作电位，表明局部 5-HT 释放缺乏自抑制作用。我们的结果支持这样的假设：5-HT 系统由具有不同适应能力的血清素能神经元亚群组成。
5-HT neurons are topographically organized in the hindbrain, and have been implicated in the etiology and treatment of psychiatric diseases such as depression and anxiety. Early studies suggested that the raphe 5-HT neurons were a homogeneous population showing similar electrical properties, and feedback inhibition mediated by 5-HT1A autoreceptors. We utilized histochemistry techniques in ePet1-eGFP and 5-HT1A-iCre/R26R mice to show that a subpopulation of 5-HT neurons do not express the somatodendritic 5-HT1A autoreceptor mRNA. In addition, we performed patch-clamp recordings followed by single-cell PCR in ePet1-eGFP mice. From 134 recorded 5-HT neurons located in the dorsal, lateral, and median raphe, we found lack of 5-HT1A mRNA expression in 22 cells, evenly distributed across raphe subfields. We compared the cellular characteristics of these neuronal types and found no difference in passive membrane properties and general excitability. However, when injected with large depolarizing current, 5-HT1A-negative neurons fired more action potentials, suggesting a lack of autoinhibitory action of local 5-HT release. Our results support the hypothesis that the 5-HT system is composed of subpopulations of serotonergic neurons with different capacity for adaptation.