Effect of Scutellariae Radix extract on experimental dextran-sulfate sodium-induced colitis in rats
Effect of Scutellariae Radix extract on experimental dextran-sulfate sodium-induced colitis in rats
复制标题
DOI：
10.3748/wjg.v13.i42.5605
复制
发表时间：
2007-11-14
影响因子：
4.3
通讯作者：
Ko, Wing-Hung
中科院分区：
文献类型：
--
作者：
Chung, Ho-Lam;Yue, Grace Gar-Lee;Ko, Wing-Hung
目的：目的：观察黄芪提取物（SRE）对葡聚糖硫酸钠（DSS）诱导的大鼠溃疡性结肠炎（UC）的影响。给药大鼠经口接受4%DSS和SRE（100 mg/kg/天）。对照组大鼠仅接受自来水或SRE。每天测定肉眼评估，包括体重变化、粪便潜血和粪便硬度。在指定的时间，处死大鼠并取出整个结肠。测量结肠长度和髓过氧化物酶（MPO）活性。通过形态学和组织学评估对结肠炎的严重程度进行分级。结肠粘膜的离子转运活性进行了评估，通过电生理technique.RESULTS：大鼠口服给药的4%DSS定期开发结肠炎的临床和肉眼可见的迹象。SRE治疗缓解了症状，包括体重减轻、结肠缩短和溃疡。SRE的给药也显著减轻DSS引起的组织学损伤。此外，结肠粘膜对毛喉素的Isc反应。在诱发结肠炎后受到抑制。刺激的离子转运活动的DSS-大鼠用SRE治疗显示显着改善分泌responsibility.CONCLUSION：SRE是有效的治疗急性DSS诱导的溃疡性结肠炎，减少临床疾病，改善宏观和组织学损伤评分，并提高正常结肠分泌功能的恢复。(c)2007年WJG。All rights reserved.
AIM: To investigate the effect of Scutellariae Radix extract (SRE) on ulcerative colitis (UC) in rats induced by dextran-sulfate sodium (DSS).METHODS: Colitis was induced in male Sprague-Dawley (SD) rats (170-180 g) by 4% dextran sulfate sodium (DSS, wt/v; MW 54000) in drinking water for 8 d. The treated rats received 4% DSS and SRE orally (100 mg/kg per day). Control rats received either tap water or SRE only. Macroscopic assessment which, included body weight changes, fecal occult blood and stool consistency were determined daily. At the appointed time, the rats were sacrificed and the entire colons were removed. The colon length and the myeloperoxidase (MPO) activity were measured. The severity of colitis was graded by morphological and histological assessments. The ion transport activity of the colonic mucosa was assessed by electrophysiological technique.RESULTS: Rats treated with oral administration of 4% DSS regularly developed clinical and macroscopic signs of colitis. Treatment with SRE relieved the symptoms, including the reduction in body weight, shortening and ulceration of the colon. Administration of SRE also significantly reduced the histological damage induced by DSS. Moreover, the Isc responses of the colonic mucosa to forskolin. were suppressed after the induction of colitis. The stimulated ion transport activity of DSS-rats treated with SRE displayed significant improvement in the secretory responsiveness.CONCLUSION: SRE was effective in treating acute DSS-induced ulcerative colitis, as gauged by reduced clinical disease, improved macroscopic and histological damage scores, and enhanced recovery of normal colonic secretory function. (c) 2007 WJG. All rights reserved.