Further data on the structure of brown seaweed fucans: relationships with anticoagulant activity
Further data on the structure of brown seaweed fucans: relationships with anticoagulant activity
复制标题
DOI：
10.1016/s0008-6215(99)00127-5
复制
发表时间：
1999-06-30
影响因子：
3.1
通讯作者：
Boisson-Vidal, C
中科院分区：
文献类型：
--
作者：
Chevolot, L;Foucault, A;Boisson-Vidal, C
比较了从褐藻Ascophyllum nodosum中提取的粗岩藻聚糖经部分水解或自由基解聚得到的各种低分子量岩藻聚糖的组成、分子量、抗凝血活性和核磁共振波谱。聚焦单元主要在O-2处被发现硫酸化，在O-3处较少，在O-4处只有少量硫酸化，这与先前发表的其他棕色海藻中褐藻糖聚糖的结果相反，并且首次观察到聚焦2,3 - o -二硫酸残留。由于磺化模式排除了α -（1—> 2）连接的聚焦骨架，并且发现了高比例的α -（1—> 4）连接，因此似乎需要修改岩藻聚糖结构的概念。抗凝血活性显然不仅与先前确定的分子量和硫酸化含量有关，而且（更准确地）与2- o -硫酸化和2,3- o -硫酸化水平有关。1999爱思唯尔科学有限公司版权所有。
The composition, molecular weight (MW), anticoagulant activity and nuclear magnetic resonance spectra of various low-molecular-weight fucans (LMWFs) obtained by partial hydrolysis or radical depolymerization of a crude fucoidan extracted from the brown seaweed Ascophyllum nodosum are compared. Fucose units were found mainly sulfated at O-2, to a lesser extent at O-3, and only slightly at O-4, contrary to previously published results for fucoidans from other brown seaweeds, and fucose 2, 3-O-disulfate residues were observed for the first time. As the sulfation pattern excluded an alpha-(1 --> 2)-linked fucose backbone and a high proportion of alpha-(1 --> 4) linkages was found, it would appear that the concept of fucoidan structure needs to be revised. Anticoagulant activity is apparently related not only to MW and sulfation content, as previously determined, but also (and more precisely) to 2-O-sulfation and 2,3-O-disulfation levels. (C) 1999 Elsevier Science Ltd. All rights reserved.