A radiometric microbiological assay for pantothenic acid in biological fluids.
A radiometric microbiological assay for pantothenic acid in biological fluids.
复制标题
生物体液中泛酸的放射性微生物测定。
DOI:
10.1016/0003-2697(89)90357-6
复制
发表时间:
1989
影响因子:
2.9
通讯作者:
Guilarte,TR
中科院分区:
文献类型:
--
作者:
Guilarte,TR
A semi-automated radiometric-microbiological assay (RMA) was developed and applied for the analysis of pantothenic acid in human blood and milk. The RMA is based on the measurement of14CO2produced from the metabolism of l-[1-14C]methionine or l-[1-14C]valine by the yeast Kloeckera brevis in the presence of pantothenic acid. The assay was shown to be sensitive and specific for the analysis of this vitamin in biological fluids.