A radiometric microbiological assay for pantothenic acid in biological fluids.

A radiometric microbiological assay for pantothenic acid in biological fluids.
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生物体液中泛酸的放射性微生物测定。

DOI:
10.1016/0003-2697(89)90357-6
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发表时间:
1989
影响因子:
2.9
通讯作者:
Guilarte,TR
Guilarte,TR
中科院分区:
生物学4区
文献类型:
--
作者:
Guilarte,TR

文献摘要

被引文献

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建立了一种半自动化的放射性微生物学分析方法(RMA),并应用于人血和乳汁中泛酸的分析。RMA是基于在泛酸存在下,由酵母Kloeckera brevis代谢l-[1- 14 C]蛋氨酸或l-[1- 14 C]缬氨酸产生的14 CO2的测量。该测定法被证明是敏感和具体的分析,这种维生素的生物液体。
A semi-automated radiometric-microbiological assay (RMA) was developed and applied for the analysis of pantothenic acid in human blood and milk. The RMA is based on the measurement of14CO2produced from the metabolism of l-[1-14C]methionine or l-[1-14C]valine by the yeast Kloeckera brevis in the presence of pantothenic acid. The assay was shown to be sensitive and specific for the analysis of this vitamin in biological fluids.