Tetrodotoxin concentrations within a clutch and across embryonic development in eggs of the rough-skinned newts (Taricha granulosa)
Tetrodotoxin concentrations within a clutch and across embryonic development in eggs of the rough-skinned newts (Taricha granulosa)
复制标题
DOI：
10.1016/j.toxicon.2014.08.060
复制
发表时间：
2014-11-01
期刊：
影响因子：
2.8
通讯作者：
Brodie, Edmund D., Jr.
中科院分区：
文献类型：
--
作者：
Gall, Brian G.;Stokes, Amber N.;Brodie, Edmund D., Jr.
河豚毒素是一种神秘的神经毒素，存在于多种生物体中。不幸的是，河豚毒素（TTX）的毒性在生活史阶段的大多数生物体知之甚少。糙皮蝾螈（Taricha granulosa）拥有已知最大数量的TTX的任何生物体和许多研究已经开始阐明这些模式在这个物种。我们进行了一系列的研究，以回答以下问题：（1）卵从一个单一的女性的离合器不同的毒性？(2)TTX浓度在胚胎发育过程中会发生变化吗？（3）蝾螈卵的胶质层是否含有TTX？我们发现，蝾螈卵中TTX的含量取决于卵在窝卵中的相对“位置”;在窝卵开始时沉积的卵比在窝卵结束时沉积的卵含有更多的TTX。在发育期间，卵毒性保持一致，直至孵化。果冻外套含有少量的TTX，但这些与胚胎的毒性无关。这些结果澄清了几个长期以来的解释胚胎毒性，并继续阐明这种两栖动物河豚毒素毒性的生活史模式。(C)2014爱思唯尔有限公司版权所有。
Tetrodotoxin is an enigmatic neurotoxin that is found in a wide-variety of organisms. Unfortunately, tetrodotoxin (TTX) toxicity across life-history stages is poorly understood in most organisms. Rough-skinned newts (Taricha granulosa) possess the greatest known quantities of TTX of any organism and numerous studies have begun to elucidate these patterns in this species. We conducted a series of studies to answer the following questions: (1) do eggs from a single female's clutch vary in toxicity? (2) does TTX concentration change during embryonic development? and (3) does the jelly coat from newt eggs possess TTX? We found that the amount of TTX in newt eggs depended on the relative "position" of the egg within a clutch; eggs deposited at the beginning of the clutch had substantially more TTX than those at the end. During development egg toxicity remained consistent until hatching. The jelly coat contained small quantities of TTX, but these were not correlated with the toxicity of the embryo. These results clarify several long-held interpretations about embryo toxicity and continue to elucidate the life-history patterns of tetrodotoxin toxicity in this amphibian. (C) 2014 Elsevier Ltd. All rights reserved.