WOUND-INDUCED CHANGES IN ROOT AND SHOOT JASMONIC ACID POOLS CORRELATE WITH INDUCED NICOTINE SYNTHESIS IN NICOTIANA-SYLVESTRIS SPEGAZZINI AND COMES
WOUND-INDUCED CHANGES IN ROOT AND SHOOT JASMONIC ACID POOLS CORRELATE WITH INDUCED NICOTINE SYNTHESIS IN NICOTIANA-SYLVESTRIS SPEGAZZINI AND COMES
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DOI：
10.1007/bf02066250
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发表时间：
1994-08-01
影响因子：
2.3
通讯作者：
OHNMEISS, TE
中科院分区：
文献类型：
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作者：
BALDWIN, IT;SCHMELZ, EA;OHNMEISS, TE
烟草（Nicotiana sylvestris Spegazzini和Comes）（茄科）的植食动物对叶片的损害产生了一种损害信号，这种信号显著增加了根部从头烟碱的合成。合成的增加导致全植物尼古丁库的增加，这反过来使植物对进一步的草食动物攻击更具抵抗力。由于信号产生和对信号的响应发生在广泛分离的组织中，因此可以研究不同损伤信号离开受损叶片的速度。我们建议，电损伤信号应该比化学损伤信号更快地离开叶片（小于60分钟）。切除一片叶子诱导一个较小的增加尼古丁的生产比穿刺损伤，所以我们检查了我们的命题切除以前穿刺后1，60，960分钟叶穿刺和量化诱导的全植物尼古丁池6天后，当诱导的尼古丁生产已达到最大值。显着诱导尼古丁的生产发生，只有当穿刺后超过1小时，这是一个缓慢移动的化学信号，而不是快速移动的电信号的特性相一致的穿刺叶切除。我们探索的化学信号的性质，并表明，添加90杯或更多的茉莉酸甲酯（MJ）在水溶液中的水培植物的根诱导从头尼古丁合成（NO3）-N-15以类似的方式诱导叶损伤。我们研究的假设，茉莉酸（JA）的功能，从地上部到根的损害信号的传递。使用GC-MS技术定量全株JA库，我们证明叶片损伤迅速（
Leaf damage by herbivores in Nicotiana sylvestris Spegazzini and Comes (Solanaceae) produces a damage signal that dramatically increases de novo nicotine synthesis in the roots. The increased synthesis leads to increases in whole-plant nicotine pools, which in tum make plants more resistant to further herbivore attack. Because signal production and the response to the signal occur in widely separated tissues, the speed with which different damage signals exit a damaged leaf can be studied. We propose that electrical damage signals should exit a leaf faster (less than 60 min) than chemical damage signals. Excision of a leaf induces a smaller increase in nicotine production than does puncture damage, so we examined our proposition by excising previously punctured leaves at 1, 60, and 960 min after leaf puncture and quantifying the induced whole-plant nicotine pools six days later when the induced nicotine production had reached a maximum. Significant induced nicotine production occurred only if punctured leaves were excised more than 1 hr after puncture, which is consistent with the characteristics of a slow-moving chemical signal rather than a fast-moving electrical signal. We explore the nature of the chemical signal and demonstrate that additions of 90 mug or more of methyl jasmonate (MJ) in an aqueous solution to the roots of hydroponically grown plants induce de novo nicotine synthesis from (NO3)-N-15 in a manner similar to that induced by leaf damage. We examine the hypothesis that jasmonic acid (JA) functions in the transfer of the damage signal from shoot to root. Using GC-MS techniques to quantify whole-plant JA pools, we demonstrate that leaf damage rapidly (