Genetic identification of a putative vinyl chloride reductase in Dehalococcoides sp strain BAV1
Genetic identification of a putative vinyl chloride reductase in Dehalococcoides sp strain BAV1
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DOI：
10.1128/aem.70.10.6347-6351.2004
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发表时间：
2004-10-01
影响因子：
4.4
通讯作者：
Löffler, FE
中科院分区：
文献类型：
--
作者：
Krajmalnik-Brown, R;Hölscher, T;Löffler, FE
Dehalocococcoides sp.菌株BAV 1将生长与氯乙烯（VC）还原脱氯生成乙烯相结合。设计针对还原脱卤酶（RDase）基因保守区域的简并引物，并用于PCR扩增推定的RDase基因从BAV 1菌株。7个独特的RDase基因片段进行了鉴定。VC生长的BAV 1培养物的转录分析表明，bvcA参与VC还原脱氯，并获得完整的序列bvcA。bvcA是不存在的Dehalococcoides分离，未能呼吸VC，但在四个8 VC呼吸混合培养物中检测到。
Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were identified. Transcription analysis of VC-grown BAV1 cultures suggested that bvcA was involved in VC reductive dechlorination, and the complete sequence of bvcA was obtained. bvcA was absent in Dehalococcoides isolates that failed to respire VC, yet was detected in four of eight VC-respiring mixed cultures.