Structural Differences in Meibum From Donors After Hematopoietic Stem Cell Transplantations.
Structural Differences in Meibum From Donors After Hematopoietic Stem Cell Transplantations.
复制标题
造血干细胞移植后供体睑脂的结构差异。
DOI：
10.1097/ico.0000000000001935
复制
发表时间：
2019
期刊：
影响因子：
2.8
通讯作者：
Borchman,Douglas
中科院分区：
文献类型：
--
作者：
Ramasubramanian,Aparna;Blackburn,Ryan;Yeo,Heegook;Sledge,SamiyyahM;Gully,ZaharaN;Singh,Sharika;Mehta,Sanya;Mehta,Aakash;Yappert,MartaC;Borchman,Douglas
目的：睑脂被认为是保护眼睛的泪液的关键组分，并且在进行造血干细胞移植（HSCT）的患者群体中尚未广泛研究睑脂的构象变化。本研究的目的是确定可能的脂质构象的变化，在睑的患者HSCT。方法：参与者谁HSCT随机抽样这项前瞻性的比较研究。对照组没有干眼，也没有接受过同种异体或自体干细胞移植。结果：采集了36例正常人（Mc）和22例HSCT患者（M-HSCT）的双眼睑脂。基于性别或种族的相变参数之间没有显著差异。以下是显著差异M-c与M-HSCT的参数之间存在显著性差异（P< 0.0001）：在33.4 ℃下，脂质顺序（%反式）从40（1）增加到54（2），协同性从7.9（0.4）至5.4（0.3）时，相变温度（o C）从30.3（0.4）增加到34.2（0.9），相变幅度（cm-1）从4.0（0.1）增加到4.7（0.5）（平均标准误差）。从接受HSCT的患者中观察到的睑脂脂质构象的变化表明干细胞移植后睑脂组成发生变化，临床医生应考虑治疗睑板腺以改善眼表。
Purpose:Meibum is considered to be a key component of tears that serve to protect the eye, and conformational changes in meibum have not been studied extensively within the population of patients who had hematopoietic stem cell transplantation (HSCT). The aim of this study was to determine possible lipid conformational changes in the meibum of patients who had HSCT.Methods:Participants who had HSCT were randomly sampled for this prospective comparative study. Control participants did not have dry eye or had not undergone allogeneic or autologous stem cell transplantation. Fourier-transform infrared spectroscopy was used to measure meibum phase transition.Results:Meibum was collected from both eyes of 36 donors without dry eye (M c) and from 22 patients who had undergone HSCT (M HSCT). There were no significant differences between the phase transition parameters based on gender or race. The following were the significant differences (P< 0.0001) between the parameters for M c compared with M HSCT: lipid order (% trans) at 33.4 C increased from 40 (1) to 54 (2), cooperativity decreased from 7.9 (0.4) to 5.4 (0.3), the phase transition temperature (o C) increased from 30.3 (0.4) to 34.2 (0.9), and the magnitude of the phase transition (cm− 1) increased from 4.0 (0.1) to 4.7 (0.5)(standard error of the mean).Conclusions:Conformational and thermodynamic differences were observed between M c and M HSCT. The changes observed in the lipid conformation of meibum from patients receiving HSCTs suggest that meibum composition changes after stem cell transplantation, and clinicians should consider treating the meibomian glands to improve the ocular surface.