Ascorbic acid: an endogenous inhibitor of isolated Na+,K+-ATPase.
Ascorbic acid: an endogenous inhibitor of isolated Na+,K+-ATPase.
复制标题
抗坏血酸：分离的 Na ,K -ATP 酶的内源性抑制剂。
DOI：
10.1016/0006-2952(85)90537-4
复制
发表时间：
1985
影响因子：
5.8
通讯作者：
Sato,PH
中科院分区：
文献类型：
--
作者：
Ng,YC;Akera,T;Han,CS;Braselton,WE;Kennedy,RH;Temma,K;Brody,TM;Sato,PH
During attempts to isolate and identify an endogenous ligand for the glycoside binding sites on Na+,K+-ATPase, bovine adrenal glands were found to contain a potent inhibitor of isolated Na+,K+-ATPase. The inhibitory principle was extracted from adrenal cortex, following homogenization in NaHCO3solution and separation on a Sephadex G-10 column. The active principle was recovered from a fraction which eluted from the column after the3H2O peak. The extract inhibited isolated Na+,K+-ATPase and the specific [3H]ouabain binding reaction. Sensitivity of the enzyme to the inhibitory action of the extract was species and tissue dependent; however, the pattern and the magnitude of the sensitivity were different from those of the digitalis glycosides. Moreover, the inhibitory principle failed to inhibit sodium pump activity, estimated from ouabain inhibitable86Rb+uptake by guinea pig brain slices. The activity of the extract to inhibit isolated Na+,K+-ATPase was stable under acidic condition but was lost rapidly at neutral pH, and could be eliminated by EDTA. In an acidic medium, the inhibitory principle had an absorption maximum at 244 nm which shifted to 264 nm and decayed rapidly at neutral pH. By using mass spectrometry, the principle was identified to be ascorbic acid, which has been shown previously to inhibit isolated Na+,K+-ATPase under appropriate conditions. Because ascorbic acid was incapable of inhibiting the sodium pump in intact cells, this inhibitor of the isolated enzyme does not appears to be the endogenous ligand which regulates sodium pump activityin vivo.
