The Amaryllidaceae alkaloids: biosynthesis and methods for enzyme discovery.
The Amaryllidaceae alkaloids: biosynthesis and methods for enzyme discovery.
复制标题
杏仁核生物碱：酶发现的生物合成和方法。
DOI：
10.1007/s11101-015-9451-z
复制
发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Kutchan TM
中科院分区：
文献类型：
--
作者：
Kilgore MB;Kutchan TM
石蒜科生物碱是具有巨大治疗前景的丰富的次生代谢物的一个例子。在这一组中鉴定具有300多个已知结构的新化合物仍然是一个活跃的研究领域。去甲肾上腺素4‘-O-甲基转移酶(N4OMT)是石蒜科生物碱生物合成酶，近年来对水仙转录本的组装进行了研究。AFF。石蒜和石蒜突出了利用新技术发现石蒜科生物碱合成基因的潜力。最近感兴趣的技术进展包括在酶学、下一代测序、基因修饰、核磁共振(核磁共振)和质谱学(MS)方面的进展。
Amaryllidaceae alkaloids are an example of the vast diversity of secondary metabolites with great therapeutic promise. The identification of novel compounds in this group with over 300 known structures continues to be an area of active study. The recent identification of norbelladine 4′-O-methyltransferase (N4OMT), an Amaryllidaceae alkaloid biosynthetic enzyme, and the assembly of transcriptomes for Narcissus sp. aff. pseudonarcissus and Lycoris aurea highlight the potential for discovery of Amaryllidaceae alkaloid biosynthetic genes with new technologies. Recent technical advances of interest include those in enzymology, next generation sequencing, genetic modification, nuclear magnetic resonance spectroscopy (NMR), and mass spectrometry (MS).