Formation of low molecular weight oligomers from chitin and chitosan stimulated by plasma-assisted processes
Formation of low molecular weight oligomers from chitin and chitosan stimulated by plasma-assisted processes
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DOI：
10.1016/j.carbpol.2017.01.026
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发表时间：
2017-05-01
影响因子：
11.2
通讯作者：
Miasnikov, V.
中科院分区：
文献类型：
--
作者：
Vasilieva, T.;Sigarev, A.;Miasnikov, V.
研究了电子束等离子体（EBP）对甲壳素和壳聚糖固体粉末的可控降解。以蟹壳甲壳素和壳聚糖为原料。利用电子束注入气体介质产生非平衡低温EBP。甲壳素和壳聚糖经EBP处理后形成分子量为800-2000 Da、多分散性为1.5-2.5的壳寡糖。原始聚合物中的β-1，4糖苷键在EBP中产生的活性氧物质的作用下降解。壳聚糖的低分子量产物在最小抑制浓度500 mcg/ml时抑制各种酵母样和丝状真菌的生长。通过优化处理条件和使用特殊的反应体积形成技术，在2 min内获得95%的壳低聚糖收率，而常规的化学水解通常需要数天。EBP刺激的水解是有希望的有效的多糖降解，可以与传统的技术竞争。(C)2017爱思唯尔有限公司版权所有
The controlled degradation of solid powders of chitin and chitosan stimulated by electron-beam plasma (EBP) was experimentally studied. Crab shell chitin and chitosan were used as original substances. The non-equilibrium low temperature EBP was generated by injecting an electron beam into a gaseous medium. Chitooligosaccharides with M-w = 800-2000 Da and polydispersion 1.5-2.5 were formed due to the EBP-treatment of chitin and chitosan. The beta-1,4 glycosidic bounds in original polymers degrade under the action of active oxygen species produced in the EBP. Low molecular weight products of chitosan inhibited the growth of various yeast-like and filamentous fungi at minimum inhibitory concentration 500 mcg/ml. By optimizing the treatment conditions and using special techniques of reaction volume formation the 95% yield of chitooligosaccharides was obtained after 2 min whereas the conventional chemical hydrolysis usually takes several days. The EBP-stimulated hydrolysis is promising for effective polysaccharides degradation and can be competitive with traditional technologies. (C) 2017 Elsevier Ltd. All rights reserved.