Comparison of morphological and DNA-based techniques for stomach content analyses in juvenile chum salmon Oncorhynchus keta: a case study on diet richness of juvenile fishes
Comparison of morphological and DNA-based techniques for stomach content analyses in juvenile chum salmon Oncorhynchus keta: a case study on diet richness of juvenile fishes
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幼鱼 Oncorhynchus keta 胃内容物分析的形态学和 DNA 技术比较：幼鱼饮食丰富度的案例研究
DOI：
10.1007/s12562-016-1040-6
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发表时间：
2017
影响因子：
1.9
通讯作者：
Kiyotaka Takishita
中科院分区：
文献类型：
--
作者：
Sakiko Orui Sakaguchi;Shigeru Shimamura;Yuichi Shimizu;Gen Ogawa;Yuichiro Yamada;Keiko Shimizu;Hiroaki Kasai;Hiroshi Kitazato;Yoshihiro Fujiwara;Katsunori Fujikura;Kiyotaka Takishita
为了识别海洋生物的胃内容物，通常进行形态学观察。然而，通过这种传统方法获得的结果经常是有偏差的，因为它很难检测部分消化的软体生物。为了解决这个问题，最近的研究使用基于DNA的（DNA条形码）分析来检查海洋生物的饮食广度。在这里，我们比较了猎物丰富的幼鱼形态观察和DNA为基础的分析，重点是少年chum鲑鱼Oncorhynchus凯塔。一个更高的数量的猎物类群被成功地确定使用基于DNA的分析比形态观察。然而，我们也注意到了基于DNA的分析的几个缺点，正如其他使用分子技术的饮食分析研究所报告的那样。例如，被捕食动物的消化程度可能导致对DNA检测的不同敏感性。此外，一些猎物分类群不能被精确地确定，因为一些目标生物群的序列数据在公共基因数据库中不可用。值得注意的是，基于DNA的分析也可能检测到次级猎物，因此，猎物类群的丰富度可能被高估了。因此，幼鱼的饮食分析需要仔细进行，考虑各自的优点和缺点的DNA为基础的技术和形态学。
To identify the stomach contents of marine organisms, morphological observations are commonly conducted. However, the results obtained by this traditional method are frequently biased, as it is difficult to detect partially digested, soft-bodied organisms. To resolve this, recent studies have used DNA-based (DNA barcoding) analyses to examine the diet breadth of marine organisms. Here, we compared the prey richness of juvenile fishes using morphological observations and DNA-based analyses, with a focus on juvenile chum salmonOncorhynchus keta. A higher number of prey taxa were successfully identified using DNA-based analyses than morphological observations. However, we also noticed several shortcomings of the DNA-based analyses, as reported in other diet-analysis studies that used molecular techniques. For example, the degree of digestion among prey taxa might have resulted in differential sensitivity to DNA detection. Additionally, several prey taxa could not be precisely identified, as the sequence data for some of the targeted organismal groups are unavailable in public gene databases. Remarkably, it is also possible that DNA-based analyses detected secondary prey, and therefore, the richness of prey taxa was likely overestimated. Thus, dietary analyses of juvenile fishes need to be carefully conducted, considering the respective advantages and disadvantages of DNA-based and morphological techniques.