SUBCELLULAR ENZYME BINDING AND THE REGULATION OF GLYCOLYSIS IN ANOXIC TURTLE BRAIN
SUBCELLULAR ENZYME BINDING AND THE REGULATION OF GLYCOLYSIS IN ANOXIC TURTLE BRAIN
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DOI：
10.1152/ajpregu.1992.262.3.r517
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发表时间：
1992-03-01
影响因子：
--
通讯作者：
STOREY, KB
中科院分区：
文献类型：
--
作者：
DUNCAN, JA;STOREY, KB
用三种不同的方法在淡水龟的大脑中评估了糖酵解酶与细胞颗粒分数的关系。每种方法都表明，八种酶中的每一种都有很大比例的结合在大脑中。分析了环境缺氧(7℃氮气泡水浸泡5h或20h)对游离态和结合态酶分布的影响。这三种技术都显示，在缺氧期间，脑醛缩酶和甘油醛-3-磷酸脱氢酶结合的百分比显着增加，而乳酸脱氢酶或肌酸激酶结合的百分比没有变化。两种方法还显示，在缺氧期间己糖激酶、磷酸果糖激酶和磷酸甘油酸激酶结合的百分比增加。在缺氧过程中，糖酵解酶与细胞结构元素的结合增加，可能在物理上定位糖酵解途径，以促进这一ATP生成途径与ATP利用过程(如膜离子泵)之间的耦合。
The association of glycolytic enzymes with the particulate fraction of the cell was assessed in the brain of the freshwater turtle, Pseudemys scripta elegans, using three different methodologies. Each method showed that a large percentage of each of eight enzymes was bound in brain. The effect of environmental anoxia (5 or 20 h submergence in N2-bubbled water at 7-degrees-C) on the distribution of enzymes between free and bound states was analyzed. All three techniques showed a significant increase in the percentages of brain aldolase and glyceraldehyde-3-phosphate dehydrogenase bound during anoxia and no change in lactate dehydrogenase or creatine kinase binding. Two methodologies also showed an increase in the percent bound during anoxia for hexokinase, phosphofructokinase, and phosphoglycerate kinase. An increased association of glycolytic enzymes with structural elements of the cell during anoxia may physically position the glycolytic pathway to facilitate coupling between this ATP-generating pathway and ATP-utilizing processes, such as membrane ion pumps.