Cell type-selective expression of the zinc finger-containing gene Nolz-1/Zfp503 in the developing mouse striatum
Cell type-selective expression of the zinc finger-containing gene Nolz-1/Zfp503 in the developing mouse striatum
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DOI：
10.1016/j.neulet.2013.05.020
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发表时间：
2013-08-26
影响因子：
2.5
通讯作者：
Liu, Fu-Chin
中科院分区：
文献类型：
--
作者：
Ko, Hsin-An;Chen, Shih-Yun;Liu, Fu-Chin
含锌指基因Nolz-1/Zfp 503是一个发育调控的纹状体富集基因。在本研究中，我们的特点是细胞类型选择性表达模式的Nolz-1蛋白在发育中的小鼠纹状体。Nolz-1免疫反应存在于Isl-1阳性腹侧LGE（vLGE，纹状体原基）中，但不存在于Pax 6阳性背侧LGE（dLGE，非纹状体原基）中。在vLGE中，Nolz-1免疫反应在早期分化的TuJ 1阳性神经元中检测到，但在Ki 67阳性增殖祖细胞中未检测到。此外，许多Nolz-1免疫反应阳性细胞共表达Foxp 1或Foxp 2，这是纹状体投射神经元的标志物。为了进一步表征Nolz-1在含D1 R的纹状体黑质和含D2 R的纹状体苍白球投射神经元中的表达，我们使用了Drd 1-EGFP和Drd 2-EGFP转基因小鼠。在Drd 1-EGFP和Drd 2-EGFP小鼠发育中的纹状体中发现了Nolz-1和EGFP双标记神经元，表明两种纹状体投射神经元群体中均存在Nolz-1表达。值得注意的是，Nolz-1蛋白在E18.5纹状体中的Nkx2.1阳性中间神经元祖细胞、Lhx 8阳性胆碱能中间神经元祖细胞、nNOS和钙视黄蛋白阳性中间神经元中不表达。在发育中的腹侧纹状体的嗅结节和延髓核中，许多Nolz-1阳性细胞共表达Sox 1，这是腹侧纹状体的重要转录调节因子，表明Nolz-1在调节腹侧纹状体发育中的作用。最后，在出生后的下调Nolz-1在背侧纹状体，Nolz-1蛋白持续表达在嗅结节从E15.5到成年。综上所述，我们的研究表明，Nolz-1作为一个早期分化的纹状体投射神经元的标记物，Nolz-1可能调节纹状体投射神经元的发育。(C)2013爱思唯尔爱尔兰有限公司版权所有。
The zinc finger-containing gene Nolz-1/Zfp503 is a developmentally regulated striatum-enriched gene. In the present study, we characterized the cell type-selective expression pattern of Nolz-1 protein in the developing mouse striatum. Nolz-1 immunoreactivity was present in Isl-1-positive ventral LGE (vLGE, striatal primordia), but absent in Pax6-positive dorsal LGE (dLGE, non-striatal primordia). In the vLGE, Nolz-1 immunoreactivity was detected in early differentiating TuJ1-positive neurons, but not in Ki67-positive proliferating progenitor cells. Moreover, many Nolz-1-immunoreactive cells co-expressed Foxp1 or Foxp2, markers for striatal projection neurons. To further characterize Nolz-1 expression with respect to D1R-containing striatonigral and D2R-containing striatopallidal projection neurons, we used the Drd1-EGFP and Drd2-EGFP transgenic mice. Nolz-1 and EGFP double labeled neurons were found in the developing striatum of Drd1-EGFP and Drd2-EGFP mice, indicating Nolz-1 expression in both populations of striatal projection neurons. Notably, Nolz-1 protein was not expressed in Nkx2.1-positive interneuron progenitors, Lhx8-positive cholinergic interneuron progenitors, nNOS and calretinin-positive interneurons in E18.5 striatum. In the developing nucleus accumbens and olfactory tubercles of ventral striatum, many Nolz-1-positive cells co-expressed Sox1, an important transcriptional regulator for ventral striatum, suggesting a role of Nolz-1 in regulating development of the ventral striatum. Finally, in contrast to postnatal down-regulation of Nolz-1 in the dorsal striatum, Nolz-1 protein was persistently expressed in the olfactory tubercle from E15.5 to adulthood. Taken together, our study suggests that Nolz-1 serves as a marker for early differentiating striatal projection neurons and that Nolz-1 may regulate development of striatal projection neurons. (C) 2013 Elsevier Ireland Ltd. All rights reserved.