Upregulation of alveolar liquid clearance after fluid resuscitation for hemorrhagic shock in rats.
Upregulation of alveolar liquid clearance after fluid resuscitation for hemorrhagic shock in rats.
复制标题
大鼠失血性休克液体复苏后肺泡液体清除率的上调。
DOI：
10.1152/ajplung.1997.273.2.l305
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Pittet,JF
中科院分区：
文献类型：
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作者：
Modelska,K;Matthay,MA;McElroy,MC;Pittet,JF
本研究的主要目的是验证以下假设：失血性休克15分钟液体复苏后，儿茶酚胺依赖性机制会上调肺泡液体清除率。将麻醉大鼠的平均动脉压维持在35 mmHg持续15 min，并用4%白蛋白溶液复苏。通过将5%白蛋白溶液滴入一个肺后1小时内远端气隙中的蛋白浓度，在5小时后测量肺泡液体清除率。在整个研究期间，出血大鼠发生了严重的代谢性酸中毒，与血浆肾上腺素水平显著升高相关。与对照组大鼠相比，老年大鼠和复苏大鼠的肺泡液体清除率增加60%。阿米洛利（10（-4）或10（-6）M）、普萘洛尔（10（-4）M）或双侧肾上腺切除术抑制肺泡液体清除率的增加。肾上腺切除和衰老大鼠静脉注射肾上腺素可重现这种效应。因此，这些数据提供了证据，儿茶酚胺依赖性调节钠转运，保护空气对洪水后几个小时的液体复苏失血性休克。
The primary objective of this study was to test the hypothesis that a catecholamine-dependent mechanism would upregulate alveolar liquid clearance after fluid resuscitation from 15 min of hemorrhagic shock. Anesthetized rats were hemorrhaged to a mean arterial pressure of 35 mmHg for 15 min and were resuscitated with a 4% albumin solution. Alveolar liquid clearance was measured 5 h later by the concentration of protein in the distal airspaces over 1 h after instillation of a 5% albumin solution into one lung. Hemorrhaged rats developed a severe metabolic acidosis that was associated with a significant rise-in plasma epinephrine levels throughout the study. There was a 60% increase in alveolar liquid clearance in hemorrhaged and resuscitated rats compared with control rats. Amiloride (10(-4) or 10(-6) M), propranolol (10(-4) M), or bilateral adrenalectomy inhibited the increase in alveolar liquid clearance. This effect was reproduced by the intravenous administration of epinephrine in adrenalectomized and hemorrhaged rats. Thus these data provide evidence for a catecholamine-dependent regulation of sodium transport that protects the airspaces against flooding several hours after fluid resuscitation from hemorrhagic shock.